扩张力对大鼠腭中缝骨重建过程中细胞凋亡的影响

发布时间：2018-03-30 05:20

本文选题：扩张力　切入点：腭中缝　出处：《江苏医药》2014年09期

【摘要】：目的探讨扩张力对大鼠腭中缝重建新骨形成过程中细胞凋亡的影响及意义。方法 55只大鼠随机分为实验组(A组,25只)和对照组(B组,30只)。A组再均分为5个亚组:A1组,扩弓1d;A2组,扩弓2d;A3组,扩弓3d;A4组,扩弓4d并固定3d;A5组,扩弓4d并固定7d。B组再均分为6个亚组:B0组为扩弓前对照;B1组、B2组、B3组、B4组和B5组分别与实验组对应,但均不扩弓。每组5只大鼠。采用自制两眼簧后牙扩弓器扩张A组大鼠上颌腭中缝组织,用TUNEL荧光标记法检测凋亡细胞。结果 A1组细胞凋亡现象多于B1组(P0.01);A2组凋亡细胞较A1组有所减少,但仍多于B2组(P0.05);A3组凋亡细胞数比A2组有所增加,且多于B3组(P0.01);A4组和B4组以及A5组与B5组凋亡细胞的量和分布差异无统计学意义(P0.05)。结论扩张力诱导下细胞凋亡水平的改变在腭中缝组织改建新骨形成过程中有着重要的生物学意义。
[Abstract]:Objective to investigate the effect of dilatation on apoptosis during palatine suture reconstruction in rats. Methods 55 rats were randomly divided into experimental group (n = 25) and control group B (n = 30). Group A _ 2, group A _ 3, group A _ 4, group A _ (4), group A _ (4), group A _ (5) and group B _ (4) were divided into 6 subgroups: control group B _ 1, B _ 2 group, B _ 3 group, B _ 4 group and B _ 5 group, respectively, and the group B _ 5 was corresponding to the experimental group, respectively, and divided into 6 subgroups: B _ B _ 0 group, B _ 1 group, B _ 2 group, B _ 3 group and B _ 5 group, respectively. In each group, the maxillary palatal suture tissue of group A was dilated with a self-made two eye sprung posterior tooth arch expander, and the maxillary palatal suture tissue of group A was dilated. Results the number of apoptotic cells in group A 1 was higher than that in group B 1, but the number of apoptotic cells in group A 1 was higher than that in group A 2, but the number of apoptotic cells in group A 1 was higher than that in group A 2. There was no significant difference in the quantity and distribution of apoptotic cells between P0.01A4, B4, A5 and B5 groups. Conclusion the change of apoptosis level induced by expansibility is important in the process of palatal suture remodeling. The biological significance.
【作者单位】：南京医科大学第一附属医院口腔科;南京医科大学口腔医学研究所;
【基金】：国家自然科学基金(30572068) 江苏省高校优势学科建设工程资助项目(JX10231081)
【分类号】：R780.2

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