季铵盐包裹溴化银纳米复合物改性聚甲基丙烯酸甲酯基树脂的基础研究

发布时间：2018-03-30 10:58

本文选题：季铵盐　切入点：银离子　出处：《昆明医科大学》2017年硕士论文

【摘要】：[目的]评估季铵盐包裹溴化银纳米复合物(AgBr/NPVP)对聚甲基丙烯酸甲酯基树脂(Poly-methyl methacrylate(PMMA)-based dental resins,下文简称:PMMA树脂)抗菌性能、细胞毒性及机械性能的影响。[方法]通过原位沉淀法合成AgBr/NPVP,采用傅立叶红外光谱(FTIR)及1H核磁共振(1HNMR)对新合成的NPVP进行表征,X线衍射(XRD)及透射电子显微镜(TEM)对新合成的AgBr/NPVP进行表征,利用激光粒度分析仪测定AgBr/NPVP的粒径大小;运用液体稀释法测定AgBr/NPVP对白色念珠菌(C.albicans)ATCC90028的最小抑菌浓度(MIC)、最小杀菌浓度(MFC);场发射扫描电镜(Fe-SEM)观察 C.albicans ATCC90028 与 1× MFC AgBr/NPVP 混悬液接触24 h后的细胞形态变化;制备含不同质量分数AgBr/NPVP的PMMA树脂试件,运用直接接触法、死/活菌染色法评估改性PMMA树脂的抗菌性能,通过WST-8试验及FTIR分别对改性PMMA树脂细胞毒性及转化率(DC)进行检测;树脂挠曲强度(FS)、挠曲模量(FM)及维氏显微硬度分别采用万能试验机及维氏硬度计进行检测。[结果]通过原位沉淀法成功合成了 AgBr/NPVP,其粒径大小为(30.86±1.46)nm。AgBr/NPVP 对 C.albicans ATCC90028 的 MIC 及 MFC 值均为250 μg/ml。Fe-SEM 示 C.albicans ATCC90028 与 1× MFC AgBr/NPVP 混悬液接触24 h后,正常细胞形态丧失,细胞完整性破坏,胞体皱缩、内陷,部分细胞裂解成碎片。PMMA树脂随AgBr/NPVP添加量的增加,抗菌活性不断增强,老化2周后,各组抗菌率均呈稳定趋势,0.3%组抗菌率达80%以上。激光共聚焦显微镜(CLSM)观察显示,改性PMMA树脂表面粘附及存活的细菌均较空白组少。根据ISO10993-5标准,改性PMMA树脂对人牙髓细胞(HDPCs)毒性较低。改性实验各组PMMA树脂聚合24 h后,DC均达70%以上。FS及FM值实验各组与对照组间无统计学差异(p0.05),0.1%及0.2%组维氏硬度值与对照组间无显著差异(p0.05),但当AgBr/NPVP添加量为0.3%时,其维氏显微硬度值有所下降,且差异具有统计学意义(p0.05)。[结论]经适量AgBr/NPVP改性的聚甲基丙烯酸甲酯基树脂对白色念珠菌ATCC90028具有一定抗菌作用,且对其细胞毒性及理化性能无显著影响。
[Abstract]:[objective] to evaluate the effects of quaternary ammonium salt encapsulated silver bromide nanocomposite (AgBr-NPVP) on the antibacterial properties, cytotoxicity and mechanical properties of Poly-methyl methacrylate(PMMA)-based dental resinses.[methods] AgBr-r / NPVP was synthesized by in situ precipitation method. The newly synthesized NPVP was characterized by FTIR and 1H NMR). The newly synthesized AgBr/NPVP was characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM).The particle size of AgBr/NPVP was measured by laser particle size analyzer.The minimal inhibitory concentration and minimal bactericidal concentration of AgBr/NPVP against C. albicansa ATCC90028 were determined by liquid dilution method, and the morphological changes of C.albicans ATCC90028 and 1 脳 MFC AgBr/NPVP suspension were observed 24 h after contact with C.albicans ATCC90028 and 1 脳 MFC AgBr/NPVP suspension.PMMA resin samples with different mass fraction of AgBr/NPVP were prepared. The antibacterial properties of modified PMMA resin were evaluated by direct contact method and death / live bacteria staining method. The cytotoxicity and conversion rate of modified PMMA resin were detected by WST-8 test and FTIR respectively.The flexural strength, modulus of flexure and Vickers microhardness of resin were tested by universal testing machine and Vickers hardness tester respectively.[results] AgBr-P / NPVP was successfully synthesized by in situ precipitation method. The particle size of AgBr-P / NPVP was 30.86 卤1.46)nm.AgBr/NPVP and the MIC and MFC values of C.albicans ATCC90028 were 250 渭 g/ml.Fe-SEM respectively. The results showed that the normal cell morphology was lost and the cell integrity was destroyed after C.albicans ATCC90028 was exposed to 1 脳 MFC AgBr/NPVP suspension for 24 h.With the increase of AgBr/NPVP content, the antibacterial activity of some cells crumpled and trapped. After 2 weeks of aging, the antimicrobial rate of each group showed a stable trend of 80% or more.The observation of laser confocal microscope showed that the surface adhesion and survival of the modified PMMA resin were less than that of the blank group.According to ISO10993-5 standard, the modified PMMA resin is less toxic to human pulp cells.The Vickers microhardness decreased and the difference was statistically significant (P 0.05).[conclusion] AgBr/NPVP modified polymethyl methacrylate resin has a certain antibacterial effect on Candida albicans ATCC90028, and has no significant effect on its cytotoxicity and physical and chemical properties.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.1

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