RNA干扰XBP1基因表达对口腔鳞状细胞癌生物学特性的影响研究

发布时间：2018-04-10 14:55

本文选题：RNA干扰 + XBP基因　；参考：《口腔医学研究》2017年09期

【摘要】：目的:检测口腔鳞状细胞癌组织中X盒结合蛋白1(XBP1)的表达及观察抑制其表达后对人口腔鳞状细胞癌Tca8113增殖凋亡的影响,并探讨其机制。方法:RT-PCR检测42例口腔鳞状细胞癌及癌旁组织中XBP1基因的mRNA表达;NC-siRNA和XBP1-siRNA转染至Tca8113细胞内,不经任何处理的细胞作为空白对照组,48h后,CCK8实验、流式细胞仪分别检测XBP1基因转染对细胞增殖、凋亡的影响;Western blot检测Cleaved caspase3、β-catenin、Cyclin D1蛋白表达的变化。结果:口腔鳞状细胞癌中XBP1基因的mRNA表达显著高于癌旁组织(P0.01);转染XBP1-siRNA能够显著降低XBP1的蛋白表达,降低细胞的存活率,促进细胞的凋亡,上调Cleaved caspase3蛋白表达,下调β-catenin、Cyclin D1蛋白表达(P0.01)。结论:RNA干扰沉默XBP1基因表达可通过抑制Wnt/β-catenin信号通路降低Tca8113增殖及诱导细胞凋亡。
[Abstract]:Aim: to detect the expression of X-box binding protein 1 (XBP1) in oral squamous cell carcinoma (OSCC) and to investigate the effect of XBP1 on the proliferation and apoptosis of Tca8113 in human oral squamous cell carcinoma (OSCC).Methods the mRNA expression of XBP1 gene in 42 cases of oral squamous cell carcinoma and its adjacent tissues were detected by RT-PCR and transfected into Tca8113 cells. The cells without any treatment were used as control group for 48 hours.The effects of XBP1 gene transfection on cell proliferation and apoptosis were detected by flow cytometry. The expression of Cleaved caspase3 and 尾 -catenin cyclin D1 were detected by Western blot.Results: the mRNA expression of XBP1 gene in oral squamous cell carcinoma was significantly higher than that in adjacent tissues (P0.01). Transfection of XBP1-siRNA could significantly reduce the expression of XBP1 protein, decrease cell viability, promote cell apoptosis, and up-regulate the expression of Cleaved caspase3 protein.The expression of 尾 -cateninCyclin D1 protein was down-regulated (P 0.01).Conclusion the silencing of XBP1 gene expression by XBP1 interference can inhibit the proliferation of Tca8113 and induce apoptosis by inhibiting the Wnt/ 尾 -catenin signaling pathway.
【作者单位】：成都医学院临床医学院;成都医学院第一附属医院实验中心;成都医学院基础医学院;
【基金】：四川省教育厅科研项目(编号:15ZB0245)
【分类号】：R739.8

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