浓缩生长因子促进骨再生的实验研究

发布时间：2018-04-11 06:48

本文选题：珊瑚羟基磷灰石 + 浓缩生长因子　；参考：《口腔医学研究》2017年09期

【摘要】：目的:评价珊瑚羟基磷灰石(CHA)联合浓缩生长因子(CGF)修复骨缺损的效果。方法:选24只新西兰兔,随机分为3组,每组8只,在每只兔的两侧颅骨上各制备1个直径10mm圆形缺损。第1组动物两侧颅骨缺损分别植入CHA/CGF和CGF,第2组分别植入CHA/CGF和空白对照,第3组分别植入CGF和空白对照。术后6周和12周取材,通过Micro-CT及组织学观察评价骨修复效果。结果:Micro-CT观察显示,术后6周和12周,CHA/CGF组新骨形成量和骨密度最高,CGF次之,空白对照组最小(P0.05)。组织学观察显示,术后6周,在CHA/CGF组整个缺损区的CHA孔隙内有散在的新骨形成,CGF组在邻近骨床的缺损区新骨长入;术后12周,CHA/CGF组的CHA孔隙内有大量新骨形成并相互融合,部分CHA降解吸收,而CGF组的缺损区新生骨进一步向缺损中央长入,但缺损中央区新生骨质较薄或仅有结缔组织充填。结论:将CHA与CGF联合使用能有效地促进骨愈合。
[Abstract]:Aim: to evaluate the effect of coral hydroxyapatite (cha) combined with concentrated growth factor (CGF) in repairing bone defect.Methods: 24 New Zealand rabbits were randomly divided into 3 groups with 8 rabbits in each group. A circular defect of 10mm was prepared on each side of the skull of each rabbit.CHA/CGF and CGF were implanted into bilateral skull defects in group 1, CHA/CGF and blank control were implanted in group 2, and CGF and blank control were implanted in group 3.Bone repair was evaluated by Micro-CT and histological observation at 6 and 12 weeks after operation.Results after 6 and 12 weeks of operation, the bone formation and bone mineral density were the highest in Cha / CGF group, and the lowest in blank control group (P 0.05).Histological observation showed that in the CHA/CGF group there were scattered new bone formation in the CHA pore of the whole defect area at 6 weeks after operation, and a large number of new bone formation and fusion were found in the CHA pore of the che / CGF group 12 weeks after operation.Part of the CHA was degraded and absorbed, while in the CGF group, the new bone grew further to the center of the defect, but the new bone in the central area of the defect was thinner or only filled with connective tissue.Conclusion: combined use of CHA and CGF can effectively promote bone healing.
【作者单位】：南方医科大学南京临床医学院南京军区南京总医院口腔科;
【基金】：南京军区医药卫生科研基金课题(编号:11MA091) 南京军区医学科技创新课题(编号:15DX020)
【分类号】：R782

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