三七总皂苷及其诱导成骨的试验和机制

发布时间：2018-04-14 08:19

本文选题：三七总皂苷 + 成骨发生　；参考：《国际口腔医学杂志》2015年01期

【摘要】：三七总皂苷(PNS)可调节多种成体干细胞的分化,与骨形成密切相关。以微波、超声和超临界流体技术提取PNS,较传统提取时间大大缩短,提取效率大大提高。PNS的诱导成骨试验包括体外试验和体内试验。体外试验显示,PNS可明显促进骨髓基质干细胞的成骨分化及增殖;体内试验显示,PNS可使骨矿化提前。PNS既可通过调节骨形态发生蛋白、胰岛素样生长因子和白细胞介素-6等细胞因子,促进骨改建;还可通过促丝裂原激活蛋白激酶、无翅型小鼠乳房肿瘤病毒整合位点家族、核因子-κB等信号转导通路调节破骨细胞分化,促进骨形成。羟磷灰石作为支架材料具有良好的成骨作用,将其与PNS复合,PNS可定向缓慢地作用于骨缺损部位并在骨缺损组织周围始终保持一定的质量浓度,从而加速骨缺损修复。
[Abstract]:Panax notoginseng saponins PNSs can regulate the differentiation of various adult stem cells and are closely related to bone formation.The extraction time of PNSs by microwave, ultrasonic and supercritical fluid technology is much shorter than that of traditional extraction. The extraction efficiency of PNS is greatly improved, including in vitro and in vivo experiments.In vitro experiments showed that PNS could significantly promote osteogenic differentiation and proliferation of bone marrow stromal cells, and in vivo test showed that PNS could make bone mineralization advance. PNS could regulate bone morphogenetic protein (BMP).Cytokines such as insulin-like growth factor (IGF) and interleukin-6 (IL-6) promote bone remodeling.Signal transduction pathways such as nuclear factor-魏 B regulate osteoclast differentiation and promote bone formation.Hydroxyapatite as a scaffold has a good osteogenic effect. The combination of hydroxyapatite and PNS can be applied to the bone defect site and maintain a certain mass concentration around the bone defect tissue, so as to accelerate the repair of the bone defect.
【作者单位】：浙江中医药大学口腔医学院;
【基金】：浙江省中医药科学研究计划(2012ZA029)
【分类号】：R783

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