骨改建中交感神经对破骨细胞生理功能的调节作用

发布时间：2018-04-16 15:24

本文选题：交感神经 + 失交感神经支配　；参考：《福建医科大学》2014年硕士论文

【摘要】：目的：骨的新陈代谢受到神经系统支配的影响。目前大量的研究表明神经系统，特别是交感神经系统参与对骨改建的调控。其调控的媒介为成骨细胞和破骨细胞。本实验通过外科手术的方法创建失交感神经支配的大鼠模型，研究骨改建中交感神经对破骨细胞生理功能的调节作用。证明失交感神经支配可以影响破骨细胞的生理功能进而影响骨改建，为临床应用提供实验依据。材料和方法： 1、利用外科手术方法将大鼠单侧颈上神经节摘除，创建大鼠失交感神经支配的动物模型。2、拔除大鼠实验侧（失交感神经支配侧）与对照侧（交感神经支配侧）上颌第一磨牙，创建拔牙窝骨愈合的动物模型。3、分别于拔牙术后01天、03天、07天、14天和21天5个时间点处死动物，进行TRAP阳性染色破骨细胞计数及RANKL，OPG蛋白免疫组化染色。结果： 1、 TRAP染色检测结果表明：拔牙窝在各个时间点均显示实验组（失交感神经支配组）破骨细胞数目少于对照组（交感神经支配组），在03天、07天和14天时,实验组与对照组相比存在统计学差异(P0.01)。 2、 RANKL，OPG蛋白免疫组化染色的组织学观察结果表明：（1）、大鼠拔牙窝RANKL的平均光密度值在各个时间点实验组均小于对照组，01天时实验组与对照组相比无统计学差异（P0.05);在03天、07天、14天、21天时实验组与对照组相比存在统计学差异（P0.01）。（2）、大鼠OPG的平均光密度值在拔牙窝内阳性表达随着拔牙时间的增加而增强;在各个时间点实验组均大于对照组；在01天、03天、07天、14天、21天时实验组与对照组相比均存在统计学差异（P0.01）。（3）、大鼠拔牙窝内RANKL/OPG含量比值在各个时间点实验组均小于对照组，在01天、03天、07天、14天、21天时实验组与对照组相比均存在统计学差异（P0.01）。结论：本实验结果表明利用外科手术方法建立的失交感神经支配能有效地抑制颌骨骨吸收，使破骨细胞数目明显减少，破骨细胞活性明显降低，骨吸收减少，骨形成增加，从而证实了交感神经系统确实参与了对颌骨骨改建的调控，，交感神经系统调控颌骨骨改建的机制可能是通过影响破骨细胞的数量和活性来完成的。
[Abstract]:Objective:The metabolism of bone is influenced by the nervous system.A large number of studies have shown that the nervous system, especially the sympathetic nervous system, is involved in the regulation of bone remodeling.Osteoblasts and osteoclasts are the mediators of its regulation.In this study, a rat model of denervated sympathetic nerve was established by surgical operation to study the effect of sympathetic nerve on the physiological function of osteoclasts in bone remodeling.It is proved that denervation can affect the physiological function of osteoclasts and bone remodeling, which provides experimental basis for clinical application.Materials and methods:1. The unilateral superior cervical ganglion was removed by surgical method, and the animal model of denervated sympathetic nerve was established. The experimental side (the anaesthetized side) and the control side (the sympathetic innervation side) were extracted from the maxillary first molar.The animal model of bone healing was established. The animals were killed at 01d, 03d, 07d, 14d and 21d, respectively. The osteoclast count of TRAP positive staining and the immunohistochemical staining of RANKL-OPG protein were performed.Results:1. The results of TRAP staining showed that the number of osteoclasts in the experimental group was lower than that in the control group at all time points, and the number of osteoclasts in the experimental group was lower than that in the control group.There was statistical difference between the experimental group and the control group (P 0.01).2. The histological observation of RANKL OPG protein immunohistochemical staining showed that the average optical density of RANKL in the tooth extraction fossa of rats was lower than that of the control group at every time point, and there was no significant difference between the experimental group and the control group on the 01st day, and the difference was not significant between the experimental group and the control group on the 03rd day (P 0.05).There was a statistical difference between the experimental group and the control group at 14 days and 21 days. The average optical density of OPG in the tooth extraction fossa increased with the increase of extraction time.At each time point, the experimental group was larger than the control group, and the experimental group was significantly different from the control group on the 1st day, 03rd day, 07 day, 14 day and 21 day, and the ratio of RANKL/OPG content in the tooth extraction fossa was smaller in the experimental group than that in the control group at each time point, and the ratio of RANKL/OPG content in the experimental group was lower than that in the control group at each time point.There was a statistical difference between the experimental group and the control group at 01 days, 03 days, 07 days, 14 days and 21 days, and there was a significant difference between the experimental group and the control group (P 0.01).Conclusion:The results showed that the aposymphatic innervation established by surgical method could effectively inhibit the resorption of jaw bone, decrease the number of osteoclasts, decrease the activity of osteoclasts, decrease bone resorption and increase bone formation.It is proved that the sympathetic nervous system is involved in the regulation of jaw bone remodeling, and the mechanism of sympathetic nerve system regulating the reconstruction of jaw bone may be accomplished by affecting the number and activity of osteoclasts.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R782.1

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