低强度脉冲超声对不同钛表面骨髓间充干细胞成骨分化的影响

发布时间：2018-04-18 11:12

本文选题：低强度脉冲超声(LIPUS) + 骨髓间充质干细胞(BMSCs)　；参考：《实用口腔医学杂志》2017年04期

【摘要】：目的:观察低强度脉冲超声(LIPUS)对大鼠骨髓间充质干细胞(BMSCs)在钛片表面成骨分化的影响。方法:将体外培养的BMSCs分别接种于光滑钛表面和大颗粒喷砂酸蚀(SLA)钛表面,进行矿化诱导并实施超声干预和假刺激。于矿化诱导后3、7 d进行碱性磷酸酶(ALP)定量检测,14 d进行ALP染色观察;矿化诱导21 d后行茜素红染色;于矿化诱导14、21 d检测成骨相关基因及蛋白表达。结果:矿化诱导后3、7 d超声组ALP活性较对照组高(P0.05),14 d超声组与对照组相比钛片表面ALP染色明显深染;茜素红染色显示,21 d后超声组的染色更明显且形成更多的矿化结节;超声组的成骨相关蛋白成骨转录因子2、骨形成蛋白、骨桥蛋白表达增高;RT-PCR检测到成骨相关基因、ALP、成骨转录因子2、骨形成蛋白、骨桥蛋白、骨钙素、Ⅰ型胶原蛋白均有不同程度表达增高(P0.05)。结论:LIPUS可以促进光滑及SLA钛表面BMSCs的成骨分化。
[Abstract]:Aim: to observe the effect of low intensity pulsed ultrasound (LIPUS) on bone marrow mesenchymal stem cells (BMSCs) on the surface of titanium tablets.Methods: the cultured BMSCs was inoculated on the smooth surface of titanium and on the surface of large particle sand-blast acid etching (SLAs) respectively. The mineralization was induced and ultrasound intervention and false stimulation were carried out.The alkaline phosphatase (ALP) assay was performed on the 7th day after mineralization, the ALP staining was performed on 14 days, the alizarin red staining was performed on 21 days after mineralization, and the expression of osteoblast-associated genes and proteins were detected at 1421 days after mineralization induction.Results: the ALP activity of ultrasound group was significantly higher than that of the control group on the 7th day after mineralization induction compared with that of the control group, and the ALP staining on the surface of the titanium slice was significantly deeper than that in the control group, and alizarin red staining showed that the staining was more obvious and more mineralized nodules were formed in the ultrasound group after 21 days.The expression of osteogenic transcription factor 2, bone morphogenetic protein and osteopontin were increased in ultrasound group. RT-PCR detected osteoblast-associated genes such as ALP, osteogenic transcription factor 2, bone morphogenetic protein, osteopontin, osteocalcin, osteopontin, osteopontin, osteopontin and osteopontin.The expression of type I collagen was increased to some extent (P 0.05).ConclusionTwo percent Lipus can promote the osteogenic differentiation of smooth and BMSCs on the surface of SLA titanium.
【作者单位】：锦州医科大学济南军区总医院研究生培养基地;济南军区总医院口腔科;锦州医科大学附属第一医院病理科;
【基金】：国家自然科学基金(编号:61471384,81400573) 全军卫生孵育计划(编号:15QNP019) 山东省科技发展计划(编号:2014GSF118101) 济南市青年科技明星计划(编号:2013032)
【分类号】：R783.6

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