抗菌治疗对兔颊VX-2鳞癌感染性微环境中树突状细胞的影响

发布时间：2018-04-18 12:10

本文选题：抗菌治疗 + 炎症　；参考：《实用口腔医学杂志》2017年05期

【摘要】：目的:探索抗菌治疗对兔颊VX-2鳞癌感染性微环境中树突状细胞(dendritic cells,DCs)成熟及功能的影响。方法:瘤粒植入形成兔颊VX-2鳞癌后,附加机械创伤及高糖饮食获得该鳞癌的炎症模型,再分为3组(n=6),A组:颊癌炎症模型使用抗生素治疗3 d;B组:颊癌炎症模型以生理盐水代替抗生素对照;C组:单纯颊癌不做处理。3 d后收集各组肿瘤标本,制成匀浆,取上清液与正常兔外周血单核细胞共培养,流式细胞仪检测DCs表面分子HLA-DR、CD83、CD86的表达,混合淋巴细胞反应检测DCs刺激T细胞增殖的能力。结果:HLA-DR、CD83、CD86阳性率及刺激指数(stimulate index,SI)由高到低均分别为:C组A组B组(P0.05)。结论:抗菌治疗有助于感染兔颊VX-2鳞癌微环境中树突状细胞的成熟及功能恢复。
[Abstract]:Aim: to investigate the effects of antibacterial therapy on the maturation and function of dendritic cells in the infective microenvironment of rabbit buccal VX-2 squamous cell carcinoma.Methods: the inflammatory model of rabbit buccal VX-2 squamous cell carcinoma was established after the tumor was implanted into the tumor and the tumor was induced by mechanical trauma and high glucose diet.The model of buccal carcinoma was treated with antibiotics for 3 days in group B: buccal carcinoma model was treated with physiological saline instead of antibiotic control group C: buccal carcinomas were collected and homogenized after 3 days without treatment of buccal carcinoma.The supernatant was co-cultured with normal rabbit peripheral blood monocytes. The expression of HLA-DRCD83 CD86 on the surface of DCs was detected by flow cytometry and the ability of T cell proliferation stimulated by DCs was detected by mixed lymphocyte reaction.Results the positive rate of HLA-DRCD83 + CD86 and stimulating index (SI) from high to low were as follows: group A, C, group A, group B, P 0.05, respectively.Conclusion: antimicrobial therapy is helpful to the maturation and functional recovery of dendritic cells in the microenvironment of rabbit buccal VX-2 squamous cell carcinoma.
【作者单位】：贵州省人民医院口腔科;遵义医学院附属口腔医院口腔颌面外科;
【基金】：贵州省科学技术基金(编号:黔科合J字LKZ[2013]36号,黔科合J字LKZ[2011]23号) 遵义市红花岗区科学技术局项目(编号:遵红科合社字[2015]09号) 贵州省研究生工作站项目(编号:黔教研合YJSZ字[2013]08)
【分类号】：R739.8

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