fimA Ⅱ型牙龈卟啉单胞菌在根尖周感染中的分布研究

发布时间：2018-04-19 21:24

本文选题：牙龈卟啉单胞菌 + fimAII型　；参考：《上海交通大学》2014年硕士论文

【摘要】：目的：本研究采用两种牙龈卟啉单胞菌fimA II型引物，检测fimA II型牙龈卟啉单胞菌在根尖周感染中的分布情况，以及fimA II型与临床症状的相关性。材料与方法：采集135例临床原发性尖周炎感染根管样本，通过牙龈卟啉单胞菌16S rDNA特异引物PCR筛选牙龈卟啉单胞菌阳性样本，，再利用两种牙龈卟啉单胞菌fimA II型特异引物，分别检测牙龈卟啉单胞菌fimA II型在不同症状根尖周感染中的检出率,并采用卡方检验分析牙龈卟啉单胞菌fimA II型与根尖周感染临床症状的相关性。结果：牙龈卟啉单胞菌在根尖周炎感染根管中的检出率为34.8%，根据牙龈卟啉单胞菌fimA II型传统引物与新引物所获得的fimA II型牙龈卟啉单胞菌检出率无明显差异（传统引物：11.8%；新引物：12.6%）。牙龈卟啉单胞菌fimA II型的检出率与瘘管正相关（P0.05），而与自发痛、叩痛、咬合痛、肿胀之间无显著相关性（P0.05）。结论：fimA II型牙龈卟啉单胞菌在根尖周炎感染根管内的检出率较高（10%），且在牙龈卟啉单胞菌检出阳性样本中fimA II型与瘘管显著相关。
[Abstract]:Objective: to detect the distribution of fimA II type porphyromonas gingivalis in periapical infection and the correlation between fimA II and clinical symptoms by using two fimA II primers of Porphyromonas gingivalis. Materials and methods: a total of 135 cases of clinical primary apical infection root canal samples were collected. The positive samples of Porphyromonas gingivalis were screened by 16s rDNA specific primers PCR, and two fimA II specific primers were used to screen the positive samples of Porphyromonas gingivalis. The detection rate of fimA II in different symptoms of periapical infection was detected, and the correlation between fimA II and clinical symptoms of periapical infection was analyzed by chi-square test. Results: the detection rate of porphyromonas gingivalis in infected root canals was 34.8%. There was no significant difference in the detection rate of fimA II type porphyromonas between traditional primers and new primers. The new primer is: 12.6. The detection rate of fimA II was positively correlated with fistula (P0.05), but not with spontaneous pain, percussive pain, occlusal pain and swelling. Conclusion the detection rate of porphyromonas gingivalis type II in apical periodontitis is higher than that in apical periodontitis, and fimA II is significantly related to fistula in positive samples of Porphyromonas gingivalis.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.4

【共引文献】