牙龈卟啉单胞菌对人滋养层细胞增殖及凋亡的影响

发布时间：2018-04-19 21:07

本文选题：牙龈卟啉单胞菌 + 人胎盘滋养层细胞　；参考：《口腔医学研究》2016年02期

【摘要】：目的:检测牙龈卟啉单胞菌感染对人胎盘滋养层细胞(HTR-8细胞)增殖以及凋亡的影响。方法:用不同感染复数(MOI)的牙龈卟啉单胞菌体标准菌ATCC 33277体外感染HTR-8细胞,未感染牙龈卟啉单胞菌的HTR-8细胞作为空白对照组。感染后,用Cell Counting Kit(cck-8)检测HTR-8细胞增殖情况,用线粒体膜电位凋亡检测试剂(JC-1)检测HTR-8细胞凋亡情况。结果:MOI=10组,感染72h内,HTR-8细胞增殖未受抑制(P0.05)。而MOI=100组和MOI=200组,感染24h开始,HTR-8细胞增殖受到明显抑制(P0.01)。并且与MOI=100组相比,MOI=200组在感染48h(P0.05)和72h(P0.01)时,HTR-8细胞增殖明显降低。牙龈卟啉单胞菌感染24h,MOI=200组HTR-8细胞凋亡率显著高于空白对照组(P0.01)。感染48h,MOI=100组HTR-8细胞凋亡率显著高于对照组(P0.01)。MOI=10组在感染24h和48h时细胞凋亡率与空白对照组相比差异无统计学意义。结论:在体外感染模型中,牙龈卟啉单胞菌标准菌ATCC 33277感染可抑制滋养层细胞增殖,且促进其凋亡。提示早产低体重儿与牙龈卟啉单胞菌感染引起滋养层细胞增殖和凋亡失衡有关。
[Abstract]:Aim: to investigate the effects of porphyromonas gingivalis infection on the proliferation and apoptosis of human placental trophoblastic cells (HTR-8). Methods: HTR-8 cells were infected with ATCC 33277, and HTR-8 cells from uninfected Porphyromonas gingivalis were used as control group. After infection, the proliferation of HTR-8 cells was detected by Cell Counting Kitcck-8, and the apoptosis of HTR-8 cells was detected by mitochondrial membrane potential apoptosis assay (JC-1). Results in 10 groups, the proliferation of HTR-8 cells was not inhibited within 72 hours after infection. In MOI=100 group and MOI=200 group, the proliferation of HTR-8 cells was significantly inhibited at 24 h after infection. Compared with the MOI=100 group, the proliferation of HTR-8 cells was significantly decreased at 48h (P0.05) and 72h (P0.01). The apoptotic rate of HTR-8 cells in the 24 h group infected with Porphyromonas gingivalis was significantly higher than that in the control group (P 0.01). The apoptosis rate of HTR-8 cells in the 48h group was significantly higher than that in the control group (P 0.01). The apoptosis rate of the control group was not significantly different from that of the control group at 24h and 48h after infection. Conclusion: in vitro infection model, ATCC 33277 infection of Porphyromonas gingivalis can inhibit the proliferation of trophoblast cells and promote their apoptosis. The results suggest that premature low birth weight infants are related to the imbalance of trophoblast proliferation and apoptosis induced by Porphyromonas gingivalis infection.
【作者单位】：武汉大学口腔医学院·口腔生物医学工程教育部重点实验室;
【基金】：国家自然科学基金资助项目(编号:81371145)
【分类号】：R781.4

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