肝泡型包虫病和慢性牙周炎患者龈沟液中炎症因子的实验研究

发布时间：2018-04-20 00:36

本文选题：肝泡型包虫病 + 慢性牙周炎　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:分析肝泡型包虫病(hepatic alveolar echinococcosis,HAE)伴慢性牙周炎(chronic periodontitis,CP)患者龈沟液中Th细胞亚群的代表性细胞因子含量的表达和意义。方法:将受试者分为4组:健康对照组(HC组)20例,牙周炎组(CP组)20例,肝泡型包虫病组(HAE组)19例,肝泡型包虫病和牙周炎共患组(HAE+CP组)22例,收集牙周临床指数和龈沟液。采用ELISA法检测81例受试者龈沟液中Th1细胞相关细胞因子干扰素(interferon-γ,IFN-γ)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α),Th2细胞相关细胞因子白细胞介素-4(interleukin-4,IL-4)、白细胞介素-10(interleukin-10,IL-10),Th17细胞相关细胞因子白细胞介素-17(interleukin-17,IL-17),Treg细胞相关细胞因子转化生长因子-β(transforming growth factor-β,TGF-β)的含量。Pearson相关法分析Th1/Th2型细胞因子的相关性。结果:CP组的牙周临床指数PD、AL均高于HAE+CP组(P0.05),BI与HAE+CP组比较差异无统计学意义(P0.05);HAE组PD高于HC组(P0.05),AL及BI和HC组比较差异无统计学意义(P0.05);CP组龈沟液中Th1代表性细胞因子(IFN-γ、TNF-α)和Th17代表性细胞因子IL-17含量高于其余各组(P0.05),HAE组龈沟液中Th2代表性细胞因子(IL-4、IL-10)含量高于其余各组(P0.05);IFN-γ与IL-4、IL-10水平均呈负相关(P0.05);TNF-α和IL-4、IL-10水平无线性相关(P0.05)。结论:HAE患者龈沟液Th2和Treg相关细胞因子呈高表达,抑制了患者牙周炎症反应,减轻牙周组织破坏,但仍需深入研究。
[Abstract]:Objective: to investigate the expression and significance of Th cell subsets in gingival crevicular fluid (GCF) of patients with hepatic alveolar hydatidosis (alveolar) and chronic periodontitis (CPE). Methods: the subjects were divided into 4 groups: healthy control group (n = 20), periodontitis group (n = 20), hepatic alveolar hydatidosis group (n = 19), hepatic alveolar hydatid disease group (n = 22) and chronic periodontitis group (n = 22). Periodontal clinical index and gingival crevicular fluid were collected. ELISA method was used to detect interferon- 纬 -interferon- 纬 -IFN- 纬, tumor necrosis factor 伪 -tumor necrosis factor- 伪, interleukin-4 interleukin-4hIL-4, interleukin-10 interleukin-10IL-10 / Th17 in 81 patients with gingival crevicular fluid (GCF). The content of transforming growth factor- 尾 transforming growth factor- 尾 (TGF- 尾) in Treg cells was analyzed by Pearson correlation method. Pearson correlation method was used to analyze the correlation of Th1/Th2 cytokines. Results there was no significant difference in periodontal clinical index between HAE CP group and HAE CP group compared with HAE CP group. There was no significant difference in Th1 representative cytokines in gingival crevicular fluid between HAE CP group and HAE CP group. The contents of TNF- 伪 and IL-17 of Th17 were significantly higher than that of IL-4 / IL-10 in gingival crevicular fluid in the other groups (P 0.05). The levels of IFN- 纬 and IL-4 were negatively correlated with the levels of IL-4 TNF- 伪 and IL-4 IL-10 in the gingival crevicular fluid of the other groups. Conclusion the high expression of Th2 and Treg related cytokines in gingival crevicular fluid (GCF) can inhibit periodontal inflammation and alleviate periodontal tissue damage in patients with Hae, but it still needs further study.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R532.32;R781.42

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