牙齿煅烧颗粒结合富血小板纤维蛋白修复骨缺损的实验研究

发布时间：2018-04-29 00:00

本文选题：牙齿煅烧颗粒 + 富血小板纤维蛋白　；参考：《口腔医学研究》2015年11期

【摘要】：目的:通过动物实验评价富血小板纤维蛋白(Platelet-Rich Fibrin,PRF)促进牙齿煅烧颗粒修复骨缺损的早期效果。方法:6月龄新西兰兔10只,于颅顶部制备4个骨缺损,每个骨缺损作为1个观察组。A组为空白对照,不填材料;B组为对照组,填入牙齿煅烧颗粒;C组为实验组,填入牙齿煅烧颗粒与PRF混合物;D组为阳性对照组,填入牙齿煅烧颗粒与骨髓混合物。术后6周取材,通过大体观察、X线检查和组织学分析骨缺损的修复效果。结果:各组骨缺损内均未见炎症反应,新骨生成率分别为:A组(7.5±2.2)%;B组(17.6±2.3)%;C组(21.0±4.7)%;D组(23.4%±3.2)%。统计学分析发现,B、C、D组的新骨生成率显著高于A组(P0.05)。C组和D组的新骨生成率显著高于B组(P0.05)。C组和D组间的新骨生成率没有显著性差异。结论:牙齿煅烧颗粒具有良好生物相容性和骨传导性,与PRF混合后可以显著加快新骨再生的速度,促进骨缺损的修复。
[Abstract]:Aim: to evaluate the early effect of platelet-Rich fibrin (Platelet-Rich) on the restoration of bone defect by dental calcination granules. Methods Ten 6 months old New Zealand rabbits were used to prepare 4 bone defects at the top of the skull. Each bone defect was used as a blank control in observation group. Group A was used as control group, group B as control group, and group C as experimental group. The mixture of teeth calcined granules and PRF was used as the positive control group, and the mixture of teeth calcined particles and bone marrow was added. Six weeks after operation, the bone defect was repaired by X-ray examination and histological analysis. Results: no inflammatory reaction was found in the bone defect in each group. The rate of new bone formation was 7.5 卤2.2 in group A and 17.6 卤2.3 in group B respectively. Statistical analysis showed that the rate of new bone formation in group D was significantly higher than that in group A (P 0.05) and group D (P 0.05). The rate of new bone formation in group D was significantly higher than that in group B (P 0.05) and group D (P 0.05). There was no significant difference in the rate of new bone formation between group B and group D. Conclusion: the dental calcined granules have good biocompatibility and bone conduction. Mixing with PRF can significantly accelerate the rate of new bone regeneration and promote the repair of bone defect.
【作者单位】：大连医科大学口腔医学院口腔颌面外科;
【基金】：辽宁省教育厅科学研究项目(编号:L2014359)
【分类号】：R782

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