应用牙本质屏障法测定牙齿充填材料体外细胞毒性

发布时间：2018-04-29 22:03

本文选题：牙齿充填材料 + 牙髓　；参考：《牙体牙髓牙周病学杂志》2014年11期

【摘要】：目的:探讨牙本质屏障法用于牙齿充填材料体外细胞毒性试验测定的特点。方法:低速锯片切新鲜拔除的磨牙,获取紧邻牙髓的颊侧牙本质片,选择通透值相近的牙本质片作为牙本质屏障,制作细胞毒性体外测试装置,用此装置测定氧化锌丁香油水门汀、复合树脂、玻璃离子水门汀、氢氧化钙水门汀、磷酸锌水门汀及阴性对照材料和阳性对照材料的细胞毒性,同时用传统的浸提液法测定这5种材料的细胞毒性。结果:牙本质屏障法测定的5种材料的细胞相对活性(%)分别为89.1±3.6、81.8±3.7、96.4±2.8、96.2±1.8、74.5±2.8;浸提液法测定的5种材料的浸提原液细胞相对活性(%)分别为35.6±5.2、72.9±4.3、91.5±1.3、90.2±2.6、59.3±2.6。结论:牙本质屏障可以显著减少充填材料对细胞的毒性作用,牙本质屏障法更能反映充填材料的体内细胞毒性。
[Abstract]:Objective: to investigate the characteristics of dentin barrier method used in vitro cytotoxicity test of dental filling materials. Methods: the low speed saw blade was used to cut the freshly extracted molars and the buccal dentin slices adjacent to the pulp were obtained. The dentin slices with similar permeability values were selected as the dentin barrier, and the in vitro cytotoxicity test device was made. The cytotoxicity of zinc oxide eugenol cement, composite resin, glass ionomer cement, calcium hydroxide cement, zinc phosphate cement, negative control material and positive control material were determined by this device. At the same time, the cytotoxicity of the five materials was determined by traditional extraction method. Results: the cell relative activity of the five materials measured by dentin barrier method was 89.1 卤3.6N 81.8 卤3.7U 96.4 卤2.8U 96.2 卤1.8N 74.5 卤2.8, respectively, and the relative activity of the five materials was 35.6 卤5.22.92.9 卤4.31,91.5 卤1.3cu, 59.3 卤2.66.The results showed that the relative cell activity of the five materials was 35.6 卤5.22.9 卤4.31,90.2 卤2.6N 59.3 卤2.66.Results: (1) the relative activity of the 5 materials measured by the dentin barrier method was 89. 1 卤3. 6 卤3. 7, respectively. Conclusion: dentin barrier can significantly reduce the cytotoxicity of filling material, and dentin barrier method can reflect the cytotoxicity of filling material in vivo.
【作者单位】：第四军医大学口腔医院口腔材料教研室
【基金】：国家自然科学基金项目(81171002)
【分类号】：R783.1

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