基于高通量测序技术研究慢性牙周炎患者龈下刮治和根面平整术治疗前后龈下菌群的变化

发布时间：2018-04-29 21:41

本文选题：慢性牙周炎 + 龈下刮治和根面平整术　；参考：《国际口腔医学杂志》2017年03期

【摘要】：目的采用基于16S核糖体DNA(r DNA)的高通量测序技术,分析10例慢性牙周炎患者接受龈下刮治和根面平整术(SRP)治疗前后龈下菌斑多样性及相对丰度的变化,探讨应用微生物群落的构成变化作为牙周炎诊断及预后评估指标的可行性。方法选择2014年3—9月在首都医科大学附属北京口腔医院牙周科就诊的10例慢性牙周炎患者作为研究对象,在SRP治疗前及治疗后3个月分别在研究对象的同一位点采集龈下菌斑样本,提取样本基因组DNA,采用Illumina Miseq平台测序,分析各组样本从门到种各水平的菌群分布及相对丰度。结果在门水平上,共检测到16个菌门,有8个门的细菌在牙周龈下菌斑菌群结构中占主要地位(99%);在属水平上,共检测到128个不同菌属,SRP治疗后3个月坦纳菌属(Tannerella)的相对丰度较治疗前明显降低(P0.05),纤毛菌属(Leptotrichia)和链球菌属(Streptococcus)的相对丰度较治疗前明显上升(P0.05);在种水平上,6种牙周可疑致病菌被检出,SRP治疗后3个月福赛坦纳菌(Tannerella forsythia)和中间普氏菌(Prevotella intermedia)的相对丰度较治疗前明显减少(P0.05)。结论慢性牙周炎患者龈下菌群具多样性,SRP治疗前后,牙周可疑致病菌的相对丰度降低,而有益菌的相对丰度升高,SRP治疗可以明显改变龈下菌群构成。
[Abstract]:Objective to analyze the changes of subgingival plaque diversity and relative abundance in 10 patients with chronic periodontitis before and after subgingival curettage and root surface leveling using high throughput sequencing technique based on 16s ribosomal DNA(r DNA. To explore the feasibility of using microbial community changes as diagnostic and prognostic indicators for periodontitis. Methods Ten patients with chronic periodontitis who were admitted to the Department of periodontitis, Beijing Stomatology Hospital affiliated to Capital Medical University from March to September 2014 were selected as the study subjects. Subgingival plaque samples were collected at the same site before and 3 months after SRP treatment. Genomic DNA was extracted from the samples. The distribution and relative abundance of bacteria from door to species were analyzed by Illumina Miseq platform sequencing. Results A total of 16 phylum were detected at the portal level, 8 of which were dominant in the structure of subgingival plaque. A total of 128 different genera of SRP were detected. The relative abundance of Tannerella3 months after treatment was significantly lower than that of pre-treatment. The relative abundance of Leptotrichia and Streptococcus were significantly increased at seed level, and 6 species of periodontal were significantly increased at seed level. The relative abundance of Tannerella forsythia and Prevotella intermedia were significantly decreased 3 months after treatment with SRP. Conclusion the relative abundance of suspected periodontal bacteria decreased before and after treatment with SRP in patients with chronic periodontitis, but the relative abundance of beneficial bacteria increased. SRP treatment can obviously change the composition of subgingival bacteria.
【作者单位】：首都医科大学附属北京口腔医院检验科;首都医科大学附属北京口腔医院牙周科;首都医科大学附属北京友谊医院临床检验中心;
【基金】：首都临床特色应用研究(Z141107006614005) 北京口腔医院学科建设基金(13-09-17)~~
【分类号】：R781.42

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