RhoC在舌癌组织中的表达及临床意义

发布时间：2018-04-29 21:38

本文选题：RhoC + 免疫组织化学　；参考：《青岛大学》2017年硕士论文

【摘要】：研究目的:Rho(Ras homologue)全称是Ras相似物,属于小分子G蛋白超家族成员。RhoC为Rho亚家族成员之一。目前,较多的实验研究发现,Rho家族成员在乳腺癌、肺癌、胰腺癌及黑色素瘤组织中均可检测到高表达,并且Rho家族与以上肿瘤组织的侵袭、转移密切相关。而在舌癌组织与RhoC之间的相关关系的研究报道较少。本文将着重于探讨RhoC在舌癌组织及癌旁组织中与转移淋巴结及正常淋巴结组织中的表达差异情况,并分析RhoC蛋白的表达水平与临床病理特征之间是否存在相关性。探讨RhoC表达水平差异在舌癌中有何临床意义。研究方法:应用免疫组织化学(Immunohistochemistry)检测技术、蛋白质印迹法(Western Blot)检测技术对42例舌癌患者的癌组织标本、35例癌旁正常组织标本、10例转移淋巴结组织标本及10例正常淋巴结组织标本进行半定量分析检测其表达差异情况,并分析RhoC蛋白表达水平高低与临床病理特征的关系,研究RhoC蛋白表达水平高低与舌癌患者预后之间的关系。结果:舌癌组织标本中RhoC蛋白的表达水平明显高于癌旁组织、正常舌组织，，其差异具有统计学意义(P＜0.05)。RhoC蛋白在舌癌伴远处转移淋巴结组织中的表达量显著高于舌癌组织及正常淋巴结组织(P0.05)。RhoC蛋白在舌癌组织的表达无性别、年龄差异(P0.05)。RhoC蛋白在舌癌组织标本中的阳性表达率与患者的肿瘤临床分期呈正相关(P0.05)。低分化的舌癌组织标本中的RhoC蛋白的阳性表达率高于高分化的舌癌组织标本(P0.05)结论:RhoC蛋白在舌癌组织标本中的阳性表达率显著高于癌旁组织及正常舌组织,舌癌伴远处转移淋巴结组织中RhoC表达量显著高于未发生远处转移淋巴结组织及舌癌组织,RhoC蛋白表达量水平的高低与舌癌组织标本的分化程度呈正相关。RhoC蛋白的表达量高低与舌癌的发生、发展与舌癌的侵袭、转移密切相关,由此推断RhoC可能参与了舌癌的发生与进展过程。
[Abstract]:Objective: the full name of Rho (Ras homologue) is a Ras similar to a member of the small molecule G protein superfamily.RhoC as a member of the Rho subfamily. At present, many experimental studies have found that the members of the Rho family can detect high expression in breast cancer, lung cancer, pancreatic cancer and melanoma, and the invasion of the Rho family and above tumor tissues. Metastasis is closely related. There are few reports on the correlation between tongue cancer tissue and RhoC. This article will focus on the difference in expression of RhoC in the tongue cancer tissues and the metastatic lymph nodes and normal lymph nodes, and to analyze the correlation between the expression level of RhoC protein and the clinicopathological features. To investigate the clinical significance of the difference of RhoC expression level in tongue cancer. Methods: using immunohistochemistry (Immunohistochemistry) detection technique and Western blot (Western Blot) detection technique for 42 cases of tongue cancer, 35 cases of normal tissue adjacent to cancer, 10 cases of metastatic lymph node tissue and 10 normal lymph nodes. The relationship between the level of RhoC protein expression and the clinicopathological characteristics was analyzed by semi quantitative analysis, and the relationship between the level of the expression of RhoC and the prognosis of the tongue cancer patients was analyzed. Results: the expression level of RhoC protein in the tissue specimens of the tongue cancer was significantly higher than that of the para cancerous tissue, the normal tongue tissue, and the expression level of the tongue cancer tissue. The difference was statistically significant (P < 0.05) the expression of.RhoC protein in tongue cancer with distant metastatic lymph nodes was significantly higher than that of tongue cancer tissue and normal lymph node tissue (P0.05), the expression of.RhoC protein in tongue cancer tissue was no sex, age difference (P0.05) the positive expression rate of.RhoC protein in tongue cancer tissue specimens and the patient's tumor clinical The positive expression of RhoC protein in the tissue specimens of low differentiated tongue cancer was higher than that of the highly differentiated squamous cell carcinoma tissue specimens (P0.05). The positive rate of RhoC protein in the tongue carcinoma tissue specimens was significantly higher than that of the para cancerous tissue and normal tongue tissue, and the expression of RhoC in the tongue cancer with the distant metastasis lymph nodes was significantly higher than that of the normal tongue tissue. There is no distant metastasis lymph node tissue and tongue cancer tissue. The level of RhoC protein expression level is positively related to the level of.RhoC protein expression and the occurrence of tongue cancer, and the development is closely related to the invasion and metastasis of tongue cancer. Therefore, it is concluded that RhoC can be involved in the process of the development and progression of tongue cancer.

【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.86

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