放疗对人涎腺腺样囊性癌细胞的miRNA-21与细胞生物学行为的影响

发布时间：2018-05-01 07:45

本文选题：涎腺 + 腺样囊性癌　；参考：《实用口腔医学杂志》2017年06期

【摘要】：目的:研究放疗对人涎腺腺样囊性癌细胞SACC-83和SACC-LM的影响以及与miRNA-21表达的相关性。方法:用Varian 23 EX对培养中的SACC-83和SACC-LM予从0(对照)、2、6、10 Gy照射,CCK-8检测、流式细胞仪观察放射处理后SACC-83、SACC-LM的细胞增殖和凋亡的变化;使用qRT-PCR法分析不同剂量放疗后SACC-83、SACC-LM细胞miRNA-21的表达变化。结果:SACC-LM细胞放射处理48 h后增殖能力随放疗剂量的增大而降低(F=1321.646,P=0.000),早期凋亡率(F=354.484,P=0.039)、晚期凋亡率(F=254.278,P=0.042)和异倍体比例(F=1562.991,P=0.001)随着放疗剂量的增大而升高,且miRNA-21的相对表达量随放疗剂量的增大而减小(F=177.964,P=0.011);SACC-83细胞放疗48 h后6 Gy放疗组细胞增殖能力明显低于其他各放疗组(F=1537.214,P=0.013),6 Gy剂量下早期凋亡率(F=88.579,P=0.006)、晚期凋亡率(F=1391.345,P=0.033)、异倍体比例(F=250.461,P=0.004)最高,miRNA-21的相对表达量最低(F=134.868,P=0.017)。结论:miRNA-21可能与SACC-LM、SACC-83细胞的放疗敏感性呈负相关。
[Abstract]:Aim: to study the effect of radiotherapy on SACC-83 and SACC-LM in human salivary adenoid cystic carcinoma cells and its correlation with miRNA-21 expression. Methods: SACC-83 and SACC-LM in culture were detected by Varian 23EX and the expression of miRNA-21 in SACC-83 SACC-LM cells was detected by flow cytometry after radiation treatment, and the expression of miRNA-21 in SACC-83 SACC-LM cells was analyzed by qRT-PCR method. Results after 48 hours of radiation treatment, the proliferative ability of the cells decreased with the increase of radiation dose, the early apoptosis rate was 354.484%, the late stage apoptosis rate was 254.278U (0.042) and the ratio of aneuploidy (F1562.991mP0.001) increased with the increase of radiation dose. And the relative expression of miRNA-21 decreased with the increase of radiation dose. The proliferative ability of SACC-83 cells in 6 Gy radiotherapy group was significantly lower than that in other radiotherapy groups at the dose of F1537.214P0.013 ~ 6 Gy, the late apoptosis rate was 1391.345P0. 033, and the ratio of aneuploidy was 0.033. The highest relative expression level of miRNA-21 was the lowest (FF134.868) P0. 017. Conclusion the results suggest that the sensitivity of SACC-LMN SACC-83 cells to radiotherapy is negatively correlated with the fraction miRNA-21.
【作者单位】：口腔疾病与生物医学重庆市重点实验室;江苏大学附属医院;重庆市肿瘤医院肿瘤放射治疗科;重庆医科大学附属口腔医院口腔颌面外科;
【基金】：重庆市医学科研计划项目(编号:2015ZDXM019)
【分类号】：R739.8

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