应用QPCR验证口腔黏膜下纤维性变癌变分子标记物的研究

发布时间：2018-05-02 05:14

  本文选题：口腔黏膜下纤维性变 + 口腔鳞癌　； 参考：《口腔医学研究》2017年10期

【摘要】：目的:探求口腔黏膜下纤维性变(oral submucous fibrosis,OSF)癌变的特异性分子标记物,为阐明口腔黏膜下纤维性变癌变关键机制及早期诊断提供理论基础。方法:基于前期基因芯片的分析数据,筛选出10个候选基因CXCL8、CXCL1、PTPRZ1、WDR66、DKK1、COX6A2、ATP1A2、TPM1、ABCA8、MIB1。采用实时荧光定量核酸扩增检测系统(QPCR)检测在OSF癌变组织12例、非OSF癌变组织10例和正常组织8例中候选基因mRNA的表达。用2~(-ΔΔCT)法分析比较基因的相对表达量。结果:CXCL1、PTPRZ1在OSF癌变的颊粘膜组织中表达明显高于非OSF癌变鳞癌组和正常对照组(P0.05);TMP1在OSF癌变的颊粘膜组织中表达明显低于非OSF癌变鳞癌组和正常对照组(P0.05)。结论:CXCL1、PTPRZ1、TPM1基因对OSF癌变的早期诊断有意义,可望成为OSF癌变的早期诊断的分子标记物。
[Abstract]:Objective: to explore the specific molecular markers of oral submucous fibrosis (submucous) carcinogenesis and to provide a theoretical basis for elucidating the key mechanism and early diagnosis of oral submucous fibrosis carcinogenesis. Methods: ten candidate genes (CXCL8, CXCL1, PTPRZ1, WDR66, DKK1, COX6A2AT1A2TPM1, ABCA8, MIB1) were selected based on the data of gene microarray analysis. The expression of candidate gene mRNA was detected in 12 cases of OSF cancerous tissue, 10 cases of non- cancerous tissue and 8 cases of normal tissue by real-time fluorescence quantitative nucleic acid amplification system. The relative expression of the gene was analyzed and compared by the method of 2 ~ (-)-螖 螖 CT. Results the expression of OSF in buccal mucosa with OSF carcinogenesis was significantly higher than that in non-OSF carcinomatous squamous cell carcinoma and normal control group. The expression of TMP1 in OSF carcinomatous buccal mucosa was significantly lower than that in non-OSF carcinomatous buccal mucosa and normal control group (P 0.05). Conclusion the OSF TPM1 gene may be useful in the early diagnosis of OSF carcinogenesis, and it is expected to be a molecular marker for the early diagnosis of OSF carcinogenesis.
【作者单位】： 中南大学湘雅医学院附属海口医院口腔医学中心;中南大学湘雅医院口腔颌面外科;
【基金】：国家自然科学基金资助项目(编号:281260166)
【分类号】：R781.5
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本文编号：1832503