低氧对牙周膜成纤维细胞增殖和成骨分化的影响

发布时间：2018-05-03 16:48

  本文选题：牙周膜成纤维细胞 + 低氧　； 参考：《上海口腔医学》2014年04期

【摘要】：目的:观察低氧对体外培养的人牙周膜成纤维细胞(periodontal ligament cells,PDLCs)增殖与成骨分化的影响。方法:体外培养鉴定人牙周膜成纤维细胞,分别用浓度为0、100、200、400μmol/L的CoCl2作用于细胞,采用MTT法观察CoCl2对PDLCs增殖的影响,运用实时定量PCR和Western免疫印迹技术检测CoCl2模拟低氧对PDLCs成骨分化的影响。采用SPSS13.0软件包对数据进行统计学分析。结果:免疫组化染色结果证实,培养细胞为牙周膜成纤维细胞。200μmol/L及400μmol/L的CoCl2均能抑制PDLCs的增殖及碱性磷酸酶、RUNX2、Ⅰ型胶原的表达,并呈剂量依赖性。结论:氯化钴模拟的低氧环境对PDLCs增殖及成骨分化具有抑制作用。
[Abstract]:Objective: To observe the effects of hypoxia on the proliferation and osteogenic differentiation of human periodontal ligament fibroblasts (periodontal ligament cells (PDLCs)) in vitro. Methods: human periodontal ligament fibroblasts were cultured in vitro, and CoCl2 was used to observe the effect of CoCl2 on PDLCs proliferation by MTT method, and the effect of CoCl2 on PDLCs proliferation was observed by MTT method, and the effect of CoCl2 on PDLCs proliferation was observed by MTT method. Real-time quantitative PCR and Western immunoblotting were used to detect the effect of CoCl2 simulated hypoxia on the osteogenesis of PDLCs. The data were analyzed by SPSS13.0 software package. Results: the results of immunohistochemical staining showed that the proliferation and alkaline phosphorus of the cultured cells of the periodontal ligament fibroblasts.200 mu mol/L and 400 u mol/L could inhibit the proliferation and alkaline phosphorus of PDLCs. The expression of acid enzyme, RUNX2 and type I collagen was dose-dependent. Conclusion: the hypoxic environment simulated by cobalt chloride can inhibit the proliferation and osteogenic differentiation of PDLCs.

【作者单位】： 上海交通大学医学院附属第九人民医院·口腔医学院牙周病科;上海市口腔医学研究所 上海市口腔医学重点实验室;
【基金】：国家自然科学基金(81070838,81271156) 上海交通大学医工交叉研究基金(YG2011MS31)~~
【分类号】：R781.4

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