体外模拟间质液压升高对涎腺腺样囊性癌细胞增殖和侵袭能力的影响

发布时间：2018-05-03 15:45

本文选题：间质液压 + 腺样囊性癌　；参考：《华西口腔医学杂志》2014年01期

【摘要】：目的模拟肿瘤内间质液压(IFP)的施加情况,建立腺样囊性癌细胞受力模型,研究不同IFP作用下腺样囊性癌细胞ACC-2的增殖和侵袭能力的变化。方法将压力锅改装成能稳定调控气压的数控力学加压装置。体外培养ACC-2细胞,以不同的压力(7.551、7.649、7.747 kPa)处理细胞并持续不同的时间(3、6、12、24 h),以未加压的ACC-2细胞为阴性对照。用CCK-8法测定细胞的生长情况,并检测细胞Ki67的表达情况以研究细胞的增殖能力;用Transwell小室试验观察细胞的侵袭能力。结果在7.649 kPa下加压6 h后细胞的增殖能力增强,并随着加压时间的延长逐渐增强,并在加压24 h后达到最大值;细胞在加压后的侵袭能力明显提高,并在加压24 h后达到最强。结论成功建立了腺样囊性癌细胞受力模型;IFP的升高可刺激细胞增殖,提高其侵袭能力。
[Abstract]:Objective to study the effects of IFP on the proliferation and invasion of adenoid cystic carcinoma cells (ACC-2) by simulating the application of IFP. Methods the pressure cooker was refitted into a numerical control mechanical pressure device which could control the pressure stably. ACC-2 cells were cultured in vitro and treated with different pressures of 7.551 ~ 7.649 ~ 7.747 KPA for 24 h. The unpressurized ACC-2 cells were used as negative control. CCK-8 assay was used to detect cell growth and Ki67 expression in order to study cell proliferation, and Transwell chamber test was used to observe cell invasion. Results the proliferation ability of the cells was increased at 7.649 kPa for 6 h, and increased gradually with the prolongation of the compression time, and reached the maximum value after 24 h compression, and the invasion ability of the cells increased obviously after compression, and reached the highest level after 24 h compression. Conclusion the elevated IFP can stimulate cell proliferation and enhance the invasion ability of adenoid cystic carcinoma cells.
【作者单位】：口腔疾病研究国家重点实验室华西口腔医院头颈肿瘤外科(四川大学);
【基金】：高等学校博士学科点专项科研基金资助项目(20090181-110082) 国家自然科学基金资助项目(30973345,81172578)
【分类号】：R739.87

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