抑制Kv1.3表达能够降低人牙周膜成纤维细胞增殖和分化成骨能力

发布时间：2018-05-03 15:32

本文选题：Kv. + 人牙周膜成纤维细胞　；参考：《安徽医科大学学报》2017年06期

【摘要】：目的探讨Kv1.3在人牙周膜成纤维细胞(h PDLFs)增殖和分化成骨能力中的作用。方法通过体外分离并培养健康人和牙周炎患者牙周膜成纤维细胞,应用细胞免疫荧光法、Q-PCR和Western blot检测Kv1.3在牙周炎h PDLFs中表达变化;并通过si RNA沉默抑制Kv1.3表达,使用MTT和流式细胞仪观察对h PDLFs的增殖和分化成骨能力的作用。结果细胞免疫荧光结果显示与健康组比较,牙周炎组h PDLFs中Kv1.3显著低表达(P0.05);Western blot结果显示Kv1.3-si RNA能够明显抑制细胞周期蛋白D1(Cyclin D1)和C-myc蛋白表达(P0.05);流式细胞仪检测显示Kv1.3-si RNA能够显著抑制细胞G2/M期;同时,Western blot结果显示Kv1.3-si RNA明显降低碱性磷脂酶(ALP)和骨钙素(OCN)蛋白表达。结论抑制Kv1.3表达能够明显抑制h PDLFs的增殖和分化成骨能力。
[Abstract]:Objective to investigate the role of Kv1.3 in the proliferation and differentiation of human periodontal ligament fibroblasts (PDLFs). Methods periodontal ligament fibroblasts were isolated and cultured from healthy subjects and patients with periodontitis in vitro. The expression of Kv1.3 in PDLFs of periodontitis was detected by Q-PCR and Western blot, and the expression of Kv1.3 was inhibited by si RNA silencing. The effects of MTT and flow cytometry on the proliferation and differentiation of h PDLFs were observed. Results the results of cellular immunofluorescence showed that compared with the healthy group, In the periodontitis group, the expression of Kv1.3 in PDLFs was significantly lower than that in the control group. The results of Western blot showed that Kv1.3-si RNA could significantly inhibit the expression of cyclin D1(Cyclin D1) and C-myc protein, and the flow cytometry showed that Kv1.3-si RNA could significantly inhibit the G 2 / M phase of the cells. At the same time, the results of Western blot showed that Kv1.3-si RNA significantly decreased the expression of alkaline phospholipase (ALP) and osteocalcin (OCN) protein. Conclusion inhibition of Kv1.3 expression can significantly inhibit the proliferation and differentiation of h PDLFs.
【作者单位】：中国人民解放军第105医院口腔科;中国人民解放军第105医院检验科;安徽医科大学口腔医学院安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室;安徽医科大学第四附属医院口腔科;
【基金】：安徽省科技计划项目(编号:12070403062)
【分类号】：R781.4

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