DAPT对成牙骨质细胞增殖活性的影响

发布时间：2018-05-08 19:41

本文选题：DAPT + 成牙骨质细胞　；参考：《口腔医学研究》2016年02期

【摘要】：目的:探讨γ-分泌酶抑制剂DAPT对成牙骨质细胞株OCCM30的Notch信号通路是否有抑制作用,及对成牙骨质细胞增殖活性的影响,并初步探讨其作用机制。方法:γ-分泌酶抑制剂DAPT作用于OCCM30细胞,分别培养2、4、6d,采用RT-PCR检测Notch信号通路下游基因(Hes-1、Hey-1)和细胞周期相关基因(Cyclin D、Cyclin E)的表达,采用流式细胞术检测DAPT对OCCM30细胞周期的影响;DAPT作用于OCCM30细胞后,连续培养8d,采用CCK-8法测定细胞增殖活性。结果:实验组Hes-1、Hey-1、Cyclin D、Cyclin E mRNA的表达下降,G1/G0期细胞百分比增高,与对照组相比差异有统计学意义(P0.05);第2~8天,实验组细胞增殖活性(A450值)显著降低,呈浓度依赖性,且与对照组相比差异有统计学意义(P0.05)。结论:DAPT能有效抑制成牙骨质细胞株OCCM30的Notch信号通路,抑制OCCM30细胞的增殖活性,其机制可能与DAPT下调细胞周期相关基因(Cyclin D、Cyclin E)表达有关。
[Abstract]:Aim: to investigate the inhibitory effect of 纬 -secretase inhibitor (DAPT) on the Notch signal pathway of cementoblast cell line OCCM30 and the effect of 纬 -secretase inhibitor DAPT on the proliferation of cementoblast cells and its mechanism. Methods: DAPT, a 纬 -secretory enzyme inhibitor, was applied to OCCM30 cells for 6 days. RT-PCR was used to detect the expression of the downstream gene of Notch signal pathway, Hes-1Hey-1) and the cell cycle related gene, cyclin Dcyclin E, respectively. The effect of DAPT on the cell cycle of OCCM30 cells was detected by flow cytometry. After OCCM30 cells were cultured for 8 days, the proliferation activity of OCCM30 cells was measured by CCK-8 assay. Results: the percentage of G _ 1 / G _ 0 phase cells in the experimental group was significantly higher than that in the control group (P < 0.05), and on the 28th day, the proliferative activity (A450) of the experimental group was significantly decreased in a concentration-dependent manner. Compared with the control group, the difference was statistically significant (P 0.05). ConclusionDAPT can effectively inhibit the Notch signaling pathway of cementoblast cell line OCCM30 and inhibit the proliferative activity of OCCM30 cells. The mechanism may be related to the down-regulation of cell cycle related gene cyclin D cyclin E by DAPT.
【作者单位】：四川大学华西口腔医院正畸科;
【基金】：国家自然科学基金(编号:81271178) 四川省科技厅支撑项目(编号:2013SZ0043)
【分类号】：R781

【相似文献】