根管充填失败后根管内微生物的分子生物学分析

发布时间：2018-05-09 04:34

本文选题：S + rRNA　；参考：《牙体牙髓牙周病学杂志》2014年10期

【摘要】：目的:基于16S rRNA,构建治疗失败根管内微生物的16S rRNA基因文库,并通过分子生物学方法分析其微生物的多样性。方法:选取经完善根管治疗2年后失败的患牙20例,根管内取样提取细菌DNA;然后用PCR扩增细菌16S rRNA基因,并构建其基因文库;最后通过测序比对构建系统发育树以确定微生物种类。结果:PCR检测显示,所有样本中有细菌出现;20个样本中共发现15种不同的系统发育型细菌(phlylotype),分属4个细菌门(phylum):厚壁菌门(Firmicutes)、变异菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria);每个样本中检出1~6种不同的细菌系统发育型,平均每个样本中可检测到2.7种;在检出的15种细菌中,以粪肠球菌(Enterococcus faecali)的出现频次最高(60%,12/20),微小消化链球菌(Peptostreptoccus micros)次之(40%,8/20),而另外13种细菌的出现频次较低,其中有4种系统发育型细菌(Rummeliibacillus、Exiguobacterium aurantiacum、Pseudomonas veronii、phocaeicola abscessus)只出现了1次。结论:根管治疗失败主要是由多种细菌感染所致,其中以粪肠球菌的检出率最高。
[Abstract]:Aim: to construct 16s rRNA gene library of failed root canal microorganism based on 16s rRNAs and analyze its microbial diversity by molecular biology. Methods: the bacterial DNA was extracted from 20 teeth which failed after 2 years of perfect root canal treatment, and the 16s rRNA gene of bacteria was amplified by PCR and its gene library was constructed. Finally, the phylogenetic tree was constructed by sequencing. Results: the PCR test showed that, A total of 15 different phylogenetic bacteria were found in 20 samples, belonging to 4 phylumus: phylum, Proteobacteriae, Bacteroidetesa, Actinobacteria, Actinobacteria; 1, 6 different species were detected in each sample. Of the bacterial phylogeny, Of the 15 bacteria detected, Enterococcus faecalii had the highest frequency of 6012 / 20, and Peptostreptococcus microsfollowed by Peptostreptoccus micros40 / 20, while the other 13 were less frequent. Among them, four phylogenetic bacteria, Rummeliibacillusus Exiguobacterium aurantiacumum Pseudomonas veroniiphocaeicola abscessus, appeared only once. Conclusion: the failure of root canal therapy is mainly caused by a variety of bacterial infections, among which Enterococcus faecalis has the highest detection rate.
【作者单位】：合肥市口腔医院牙体牙髓科;
【基金】：安徽省年度重点科研项目(11070403040)
【分类号】：R781.05

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