碳酸饮料对乳恒牙更替期牙齿健康的影响

发布时间：2018-05-15 17:29

本文选题：碳酸盐饮料 + RANK配体　；参考：《天津医药》2014年06期

【摘要】：目的研究碳酸饮料对乳恒牙更替期牙齿健康的影响作用及机制。方法 40颗犬牙釉质样本分别采用15μL可口可乐(可口可乐组)、雪碧(雪碧组)、纯苏打水(苏打水组)和生理盐水(生理盐水组)浸泡,各10颗。测定处理后饮料中钙、磷溶出浓度;分离牙乳头干细胞,以添加可口可乐、雪碧、苏打水和生理盐水的条件培养基进行细胞培养,采用PCR法和Western blot法检测核因子-κB受体活化剂配体(RANKL)、骨保护素(OPG)和维生素D受体(VDR)的mRNA和蛋白表达水平。结果各组钙、磷溶出浓度(mmol/L)由高到低均依次为:雪碧组(3.679±0.114、0.089±0.008)、可口可乐组(2.217±0.109、0.036±0.005)、生理盐水组(0.021±0.004、0.009±0.001)和苏打水组(0.007±0.001、0.002±0.000)。各组RANKL的mRNA和蛋白表达差异均无统计学意义。苏打水组和生理盐水组OPG的mRNA和蛋白表达水平均高于可口可乐组和雪碧组(均P0.05),雪碧组与可口可乐组、生理盐水组与苏打水组间差异均无统计学意义。生理盐水组VDR的mRNA和蛋白表达水平低于可口可乐组和雪碧组,高于苏打水组(均P0.05)。结论碳酸饮料可能通过调控成骨相关基因表达及维生素D受体家族共同影响乳恒牙更替期的牙齿健康。
[Abstract]:Objective to study the effect and mechanism of carbonated beverage on the health of primary permanent teeth. Methods 40 canine enamel samples were soaked with 15 渭 L Coca cola (Coca Cola group), Sprite group (Sprite group), pure soda water (soda water group) and normal saline (normal saline group), 10 in each group. The calcium and phosphorus dissolving concentration in the treated beverage was determined, the dental papillary stem cells were isolated, and the cells were cultured on the conditioned medium supplemented with Coca-Cola, Sprite, soda water and normal saline. The expression levels of mRNA and protein of nuclear factor- 魏 B receptor activator ligand RANKL, osteoprotegerin (OPG) and vitamin D receptor (VDR) were detected by PCR and Western blot methods. Results the concentrations of calcium and phosphorus in each group ranged from high to low: Sprite group (3.679 卤0.114), Coca Cola group (2.217 卤0.109 卤0.005), saline group (0.021 卤0.004) and soda water group (0.007 卤0.001 卤0.002 卤0.000). There was no significant difference in the expression of mRNA and protein in all groups of RANKL. The expression of mRNA and protein in OPG of soda water group and saline group were higher than that of Coca-Cola group and Sprite group (P0.05). There was no significant difference between Sprite group and Coca Cola group and normal saline group and soda water group. The expression of mRNA and protein in VDR of saline group was lower than that of Coca Cola group and Sprite group, and higher than that of soda water group (all P 0.05). Conclusion carbonated beverage may affect the dental health of primary permanent teeth by regulating the expression of osteoblast related genes and vitamin D receptor family.
【作者单位】：天津市第五中心医院口腔科;天津市第五中心医院病理科;天津市第五中心医院中心实验室;
【基金】：天津市卫生局科技基金项目(项目编号:2011KZ25)
【分类号】：R788

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