葡萄糖定量法比较五种根管封闭剂的封闭性能

发布时间：2018-05-19 09:05

本文选题：根管封闭剂 + 微渗漏　；参考：《吉林大学》2014年硕士论文

【摘要】：根管治疗失败的最主要原因是根管充填后出现的微渗漏。本实验中所检测的5种根管封闭剂是目前临床上较为代表性的封闭剂，主要是氧化锌丁香油类封闭剂、树脂类封闭剂及硅酮类封闭剂。目前为止还没有对这5种封闭剂进行过根管充填后微渗漏的对比研究。目的：通过葡萄糖定量检测方法，检测比较5种根管封闭剂—EZ Fill、AHPlus、2Seal、Cortisomol、GuttaFlow的根管充填后出现的微渗漏量。材料方法：选取62颗离体单根管下颌前磨牙随机分为5组，每组包含10颗牙齿，另设阴性对照组和阳性对照组各6颗。以机用Protaper配合5.25%次氯酸钠溶液预备至F3，，然后以17%EDTA和5.25%次氯酸钠交替冲洗。分别以EZ Fill、AH Plus、2Seal封闭剂和热牙胶垂直加压法充填10颗；GuttaFlow根管充填技术充填10颗；Cortisomol封闭剂和热牙胶垂直加压各充填16颗（其中6颗作为阴性对照组）；剩余6颗仅用热牙胶垂直加压充填作为阳性对照组。将实验组和阳性组牙体表面（除根尖孔外2mm）涂两层不同颜色的指甲油，阴性组包括根尖孔在内的所有牙面涂两层指甲油，其后建立葡萄糖定量检测模型，并在1、2、4、6及8w采用葡萄糖氧化酶比色法检测渗漏出的葡萄糖浓度，并将所得数据用Excel录入计算机，spss17.0统计软件进行统计学处理与分析比较各组样本在各时间段的微渗漏量。结果：阴性对照组在各时间段均未检测到葡萄糖的渗出；阳性对照组在1w即检测到比较高浓度的葡萄糖，且表现出快速的增加趋势。在1w时Cortisomol组微渗漏数值明显大于AH Plus组，且有显著性差异（P＜0.05），2w和4w时微渗漏数值明显大于EZ Fill、AH Plus、2Seal与GuttaFlow组，且有非常显著性差异（P＜0.001），而EZ Fill、AH Plus、2Seal与GuttaFlow组之间的微渗漏量之间无显著性差异（P＞0.05）。在6w和8w时AH Plus组的微渗漏数值明显小于EZ Fill、2Seal、Cortisomol与GuttaFlow组，且有显著性差异（P＜0.05），同时，EZ Fill、2Seal、Cortisomol与GuttaFlow组之间微渗漏数值之间无统计学差异。随着时间推移5种根管封闭剂微渗漏的葡萄糖量平稳升高。结论：本实验结果显示AH Plus的早期的封闭性能与EZ Fill、2Seal和GuttaFlow封闭剂无明显差异，晚期优于其他四组封闭剂；Cortisomol早期微渗漏明显大于其他各组，较晚时其封闭性与EZ Fill、2Seal和GuttaFlow组无明显差异，与AH Plus租比较微渗漏有明显差异；而EZ Fill、2Seal和GuttaFlow组间在各阶段均无明显差异。
[Abstract]:The main reason for the failure of root canal therapy is the microleakage after root canal filling. The 5 kinds of root canal sealants detected in this experiment are the representative sealants in clinic at present, which are mainly zinc oxide clove oil sealants, resin sealants and silicone sealants. So far, there has not been a comparative study of microleakage after root canal filling with these five sealants. Objective: to detect and compare the microleakage of 5 root canal sealants-EZ Fillus AHPlus2 Sealtisomolus Cortisomolus GuttaFlow after root canal filling by quantitative detection of glucose. Materials: 62 single root canals mandibular premolars were randomly divided into 5 groups, each containing 10 teeth, and the negative control group and the positive control group each with 6 teeth. Mechanical Protaper and 5.25% sodium hypochlorite solution were used to prepare F _ 3 and then flushed alternately with 17TA and 5.25% sodium hypochlorite. 10 GuttaFlow root canal fillings were filled with EZ Fillon AH Plust2Seal sealant and hot gum vertical compression technique respectively. 10 Cortisomol sealants and 16 hot gum vertical compression were filled respectively (6 as negative control group and 6 as negative control group); the remaining 6 were filled with heat only. Gum vertical compression filling was used as the positive control group. Two layers of nail polish of different colors were applied on the surfaces of the experimental group and the positive group (except for the apical foramen), and all the teeth in the negative group, including the apical foramen, were coated with the two layers of nail polish, and the glucose quantitative detection model was established. Glucose concentration was detected by glucose oxidase colorimetry at 1w and 8w, and the data were recorded in the computer software SPSS 17.0 for statistical analysis and comparison of microleakage in each time period. Results: no exudation of glucose was detected in the negative control group and a relatively high concentration of glucose was detected in the positive control group at 1 week and showed a rapid increase trend. The microleakage value of Cortisomol group was significantly higher than that of AH Plus group at 1 week, and the microleakage value of EZ Fillar AH Plus2Seal group was significantly higher than that of EZ Fillar AH Plus2Seal group and GuttaFlow group at 4 weeks and P < 0.05 respectively. There was significant difference (P < 0. 001), but there was no significant difference in microleakage between EZ Fillus AH Plus2 Seal and GuttaFlow group (P > 0. 05). The microleakage value of AH Plus group at 6 and 8 weeks was significantly lower than that of EZ Filler2SealCortisomol group and GuttaFlow group (P < 0.05), and there was no significant difference between EZ Fillering2SealalCortisomol group and GuttaFlow group. The glucose content of microleakage of 5 root canal sealants increased steadily over time. Conclusion: the results showed that the early sealing performance of AH Plus was not significantly different from that of EZ Fill2 Seal and GuttaFlow sealants, and the early microleakage of AH Plus was significantly higher than that of other four sealants. There was no significant difference between EZ Fillering2Seal group and EZ Filler2 Seal group, but there was significant difference with AH Plus rent group in microleakage, but there was no significant difference between EZ Fill2 Seal group and GuttaFlow group in all stages.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.05

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