丹参对大鼠正畸牙牙周组织中OPG RANKL表达的影响

发布时间：2018-05-19 14:39

本文选题：丹参 + 正畸　；参考：《遵义医学院》2014年硕士论文

【摘要】：目的：研究丹参注射液对大鼠正畸牙压力侧牙周膜中RANKL和OPG表达的影响。方法：清洁级SD雄性大鼠240只，随机分为A正畸加力组，B丹参干预组，C丹参对照组，D空白对照组，AD两组与BC两组每天于左上颌第一磨牙颊侧黏膜下分别注射0.1mlPBS或者丹参注射液；在实验开始后第l、3、5、7、10、14天分批处死，测量左上颌第一磨牙近中移动距离，免疫组织化学检测RANKL和OPG的表达，TRAP染色检测破骨细胞。结果：丹参干预组自实验第5天起牙齿移动距离显著大于正畸加力组（P＜0.05）；正畸加力组与丹参干预组之间RANKL表达无明显差异（P＞0.05）；丹参干预组自实验第7天起OPG的表达显著大于正畸加力组（P＜0.05）；在实验第3天和第5天，，丹参干预组中的破骨细胞数目显著大于正畸加力组（P＜0.05）。结论：丹参注射液对大鼠正畸牙压力侧的RANKL表达无明显影响，却可以使压力侧出现更多的破骨细胞从而加速牙周改建；同时能够促使大鼠正畸牙压力侧的OPG表达上调，但不能将OPG到达峰值的时间提前，使其在加速正畸牙移动的同时维持骨量。
[Abstract]:Aim: to study the effect of Salvia miltiorrhiza injection on the expression of RANKL and OPG in orthodontic periodontal ligament of orthodontic teeth. Methods: 240 male SD rats of clean grade were randomly divided into two groups: group A, orthodontic treatment group, group B, group C, the control group of Salvia miltiorrhiza, group C, the control group D, the control group, AD and group BC, respectively, 0.1mlPBS or salvia miltiorrhiza injection were injected into the buccal mucosa of the left maxillary first molar every day. At the beginning of the experiment, the first molar of left maxillary was killed in batches on the 14th day. The distance of proximal movement of left maxillary first molar was measured. The expression of RANKL and OPG was detected by trap staining. The osteoclasts were detected by immunohistochemistry. Results: the distance of tooth movement in Danshen intervention group was significantly higher than that in orthodontic plus force group (P < 0.05), but there was no significant difference in RANKL expression between orthodontic and salvia miltiorrhiza intervention group (P > 0.05), and the expression of OPG in salvia miltiorrhiza intervention group from the 7th day of experiment was significantly higher than that of orthodontic treatment group (P < 0.05). It was larger than that in orthodontic stress group (P < 0.05), and on the 3rd and 5th day, The number of osteoclasts in salvia miltiorrhiza intervention group was significantly higher than that in orthodontic treatment group (P < 0.05). Conclusion: Salvia miltiorrhiza injection has no obvious effect on the expression of RANKL on the pressure side of orthodontic teeth, but it can make more osteoclasts appear in the pressure side of the orthodontic teeth and accelerate the periodontal remodeling. It can also promote the expression of OPG in the pressure side of orthodontic teeth. However, the peak time of OPG can not be brought forward, so that the bone mass can be maintained while accelerating orthodontic tooth movement.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.5

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