偏侧咀嚼对SD大鼠髁突软骨中DMP1表达影响的实验研究

发布时间：2018-05-20 07:26

本文选题：偏侧咀嚼 + 髁突软骨　；参考：《昆明医科大学》2017年硕士论文

【摘要】：[目的]探讨偏侧咀嚼对SD大鼠髁突软骨中DMP1表达的影响。[方法]通过拔除8周龄SD雄鼠右侧上颌所有磨牙建立偏侧咀嚼动物模型,于术后2w、4w、6w、8w取材(髁突),常规固定,脱钙,脱水,包埋、石蜡切片和染色。HE染色:观察髁突软骨及软骨下骨组织学变化,测量髁突软骨厚度,进行髁突软骨下骨骨形态计量分析(BV/TV、Tb.Th、Tb.N、Tb.Sp);免疫组织化学方法检测不同时间点SD大鼠髁突软骨中DMP1的表达变化。采用Image Pro Plus 6.0图像分析系统进行测量分析。所有数据均应用SPSS23.0统计软件进行统计学分析。[结果]1、HE染色显示实验组髁突软骨及软骨下骨都出现了病理性改变,表现为软骨厚度降低,软骨下骨骨小梁排列紊乱。髁突软骨厚度测量结果显示:术后4w、6w、8w实验组软骨厚度小于对照组(P0.05),拔牙侧与非拔牙侧组差异无统计学意义(P0.05);髁突软骨下骨骨形态计量分析结果显示:BV/TV在术后2w、4w、6w拔牙侧、非拔牙侧均低于对照组(P0.05);Tb.Th、Tb.N在术后2w拔牙侧、非拔牙侧均低于对照组(P0.05);Tb.Sp在术后2w、4w拔牙侧、非拔牙侧均高于对照组(P0.05)。拔牙侧与非拔牙侧各软骨下骨参数差异无统计学意义(P0.05)。2、免疫组化结果显示:实验组与对照组髁突软骨的DMP1都呈阳性表达,表达于成软骨细胞层和肥大细胞层的细胞核及周围胞质。实验组中拔牙侧、非拔牙侧DMP1表达在术后2w、4w、6w、8w都高于对照组(P0.05)。拔牙侧与非拔牙侧DMP1表达的差异无统计学意义(P0.05)。拔牙侧DMP1表达在术后2w与4w、4w与8w,非拔牙侧DMP1表达在术后2w与4w、4w与6w的差异有统计学意义(P0.05)。[结论]1、通过拔牙法建立的偏侧咀嚼会导致实验组SD大鼠拔牙侧和非拔牙侧髁突软骨和髁突软骨下骨出现病理性改变。髁突软骨厚度变薄,双侧髁突软骨下骨骨小梁数目、形态结构的异常以及排列的紊乱。拔牙侧与非拔牙侧髁突软骨及软骨下骨病理改变无差异。2、DMP1参与髁突软骨的生长发育,主要表达在髁突软骨成软骨细胞层和肥大层的细胞核及细胞质;偏侧咀嚼导致髁突软骨发生病理性改建,实验组髁突软骨DMP1阳性表达高于对照组,说明DMP1参与偏侧咀嚼引起的髁突软骨改建。
[Abstract]:[objective] to investigate the effect of unilateral mastication on the expression of DMP1 in condylar cartilage of SD rats. [methods] the animal model of unilateral mastication was established by pulling out all the right maxillary molars of 8-week-old SD rats. The mandibular condyle was fixed, decalcified, dehydrated and embedded at 2 weeks, 4 weeks, 6 weeks and 8 weeks after operation. Paraffin sections and staining. HE staining: the histological changes of condylar cartilage and subchondral bone were observed and the thickness of condylar cartilage was measured. The bone morphology of subcondylar cartilage was measured and the expression of DMP1 in condylar cartilage of SD rats at different time points was detected by immunohistochemical method. Image Pro Plus 6.0 image analysis system is used for measurement and analysis. All the data were analyzed by SPSS23.0 statistical software. [results] 1HE staining showed that there were pathological changes in condylar cartilage and subchondral bone in the experimental group, which showed that the thickness of cartilage decreased and the trabecular arrangement of subchondral bone was disordered. The thickness measurement of condylar cartilage showed that the thickness of cartilage in the experimental group was less than that in the control group at 4 weeks and 6 weeks after operation, but there was no significant difference between the extraction side and the non-extraction side. The non-extraction side was lower than that of the control group (P 0.05), and the non-extraction side was lower than that of the control group (P 0.05) and the non-extraction side was higher than that of the control group (P 0.05) at 2 and 4 weeks after the operation, and the non-extraction side was higher than that of the control group (P 0.05). There was no significant difference in the parameters of subchondral bone between the extraction side and the non-extraction side. The immunohistochemical results showed that the DMP1 expression was positive in condylar cartilage of the experimental group and the control group. Expressed in the nuclei and surrounding cytoplasm of chondroblasts and mast cell layers. In the experimental group, the expression of DMP1 in the extraction side and non-extraction side was significantly higher than that in the control group at 2, 4, 4, 6 and 8 weeks postoperatively. There was no significant difference in the expression of DMP1 between the extraction side and the non-extraction side (P 0.05). The expression of DMP1 in the extraction side was significantly higher than that in the non-extraction side at 2 and 4 weeks after extraction and at 4 and 8 weeks postoperatively, and there was a significant difference in the expression of DMP1 between 2 and 4 weeks after extraction and at 4 and 6 weeks postoperatively (P 0.05). [conclusion] 1. Unilateral mastication established by extraction method may lead to pathological changes of condylar cartilage and subcondylar cartilage of SD rats in experimental group. The thickness of condylar cartilage thinned, the number of bone trabeculae of bilateral condylar cartilage, the abnormality of morphology and structure, and the disorder of arrangement. There was no difference in pathological changes of condylar cartilage and subchondral bone between extraction side and non-extraction side. 2DMP1 was involved in the growth and development of condylar cartilage, mainly expressed in the nucleus and cytoplasm of chondroblast layer and hypertrophic layer of condylar cartilage. Unilateral mastication resulted in pathological remodeling of condylar cartilage. The positive expression of DMP1 in condylar cartilage in experimental group was higher than that in control group, indicating that DMP1 was involved in condylar cartilage remodeling induced by unilateral mastication.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R782.6

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