Micro-CT动态观察大鼠牙齿移动过程中微观结构的变化

发布时间：2018-05-23 17:47

本文选题：Micro-CT + 正畸牙齿移动　；参考：《上海交通大学学报(医学版)》2017年02期

【摘要】：目的·Micro-CT活体扫描动态观察不同矫治力作用下,大鼠牙齿移动过程中微观结构的变化。方法·选择8周龄雄性SD大鼠15只,随机分成3组,每组5只。对大鼠左侧上颌第一磨牙分别施加20、50、100克力(1克力=0.009 8 N)的矫治力,持续加载14 d。在加力后的0、3、7、10、14 d,用Micro-CT对大鼠进行活体扫描,测量每个时间点牙根表面陷窝体积及骨小梁结构参数。结果·加力3 d,各组牙根表面陷窝体积略有增加,第3~10日明显增加,第10日后增加缓慢;第7~14日,100克力力值组牙根表面陷窝体积显著大于其他2组(P0.05)。随着加力时间的推移,压力侧牙槽骨的骨体积分数逐渐减小,骨小梁形态变细小、数量减少,骨小梁间隙先减小后增宽,骨小梁有自板状向杆状转变的趋势,牙槽骨发生吸收,各力值组间变化趋势一致。结论·不同矫治力值都会引起牙根吸收,吸收规律表现为缓慢期、快速期、稳定期,过大的矫治力会导致更多的牙根吸收;牙齿移动过程中,压力侧牙槽骨均会发生吸收改建,其变化量与力值大小无明显相关性。
[Abstract]:Objective to observe the changes of microstructures during tooth movement in rats under different orthodontic forces by Micro-CT scanning in vivo. Methods Fifteen 8-week old male SD rats were randomly divided into 3 groups with 5 rats in each group. The left maxillary first molars of rats were subjected to the orthodontic force of 20g 50100 klystrin (0.009 8 N) for 14 days. Rats were scanned in vivo with Micro-CT for 10 ~ 14 days after applied stress. The volume of root surface trabeculae and the structure parameters of trabeculae were measured at each time point. Results on the 3rd day, the volume of root surface lacunae increased slightly in each group, increased obviously from the 3rd to 10th day, and increased slowly on the 10th day, and the volume of the root surface pit in the 714 ~ 14th KL group was significantly larger than that in the other two groups (P 0.05). The bone volume fraction of the alveolar bone on the pressure side gradually decreased, the shape of the trabecular bone became smaller and the number decreased, the space of the trabecular bone decreased first and then widened, the trabecular bone changed from plate to rod, and the alveolar bone was absorbed. The change trend of the force value groups is the same. Conclusion different orthodontic force values can cause root resorption, and the absorption patterns are slow, rapid, stable, and excessive orthodontic force will lead to more root resorption, and resorption and remodeling of alveolar bone on the pressure side during tooth movement. There was no significant correlation between the variation and the force value.
【作者单位】：重庆医科大学附属口腔医院;口腔疾病与生物医学重庆市重点实验室;重庆市高校市级口腔生物医学工程重点实验室;
【基金】：重庆市卫生局自然科学基金(2011-1-057,2012-2-132) 重庆市渝北区科学技术委员会自然科学基金[2014(社)10号]~~
【分类号】：R783.5

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