IL-23R基因多态性与口腔扁平苔癣的相关研究

发布时间：2018-05-23 19:16

本文选题：IL-23R + 基因多态性　；参考：《吉林大学》2015年硕士论文

【摘要】：口腔扁平苔藓(oral lichen planus，OLP)是常见的口腔黏膜疾病，病程迁延，不易治愈。是T细胞介导的慢性炎症性疾病，CD4+T淋巴细胞的免疫异常在其发病中起关键作用，以上皮细胞不全角化、基底层液化变性以及固有层密集的淋巴细胞浸润带为病理特征。 IL-23是针对炎症性疾病、自身免疫疾病和肿瘤研究的细胞因子[1]，作为IL-23信号传递因子的IL-23R是其复合物的两个亚基之一，在2002年被首次报道后，逐渐引起人们的重视。相关文献已报道了IL-23R在炎性疾病、自身免疫性疾病的发病机制以及恶性肿瘤的易感性里占重要地位，，但其在口腔扁平苔癣的研究几近空白。目的：研究IL-23R基因多态性与口腔扁平苔癣的相关性。方法：采用聚合酶链反应—限制性片段长度多态（PCR-RFLP）检测66例口腔扁平苔癣患者（其中糜烂型21例）和70例健康对照者的IL-23R的两个SNP位点（rs10889677和rs11465817），分析IL-23R基因多态性与口腔扁平苔癣的相关性。结果： 1.IL-23R的rs10889677、 rs11465817两个位点均检测出AA、AC、CC三种基因型。 2.rs10889677、 rs11465817位点等位基因、基因型分布频率在病例组和对照组间差异无统计学意义(P0.05)； 3.rs10889677位点基因型分布频率在糜烂型与对照组比较差异有统计学意义(P=0.024)， CC+CA基因型个体发生糜烂型的风险是AA基因型的3.115倍(95%CI:1.135-8.548)。结论： 1.受检OLP人群IL-23R基因的两个位点（rs10889677、rs11465817）均存在基因多态性。 2.IL-23R基因rs11465817位点SNP与OLP疾病易感性无明显相关性。 3.rs10889677位点存在多态性变异，可能与OLP的疾病易感性和严重程度有关。
[Abstract]:Oral lichen planus (OLP) is a common oral mucosal disease. It is a chronic inflammatory disease mediated by T cells that the immune abnormality of CD4 T lymphocytes plays a key role in the pathogenesis of the disease. It is characterized by incomplete keratosis of epithelial cells liquefaction of the basal layer and dense lymphocytic infiltrating zone of the lamina propria. IL-23 is a cytokine for the study of inflammatory diseases, autoimmune diseases and tumors. IL-23R, as a signal transduction factor of IL-23, is one of the two subunits of its complex. After it was first reported in 2002, it has attracted more and more attention. It has been reported that IL-23R plays an important role in the pathogenesis of inflammatory diseases autoimmune diseases and the susceptibility of malignant tumors. However the study of IL-23R in oral lichen planus is almost blank. Objective: To study the association between IL-23R gene polymorphism and oral lichen planus. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect two SNP loci of IL-23R in 66 patients with oral lichen planus (including 21 patients with erosive type) and 70 healthy controls. The polymorphism of IL-23R gene and oral cavity were analyzed. The association of lichen planus. Results: Rs10889677 of 1.IL-23R, three genotypes of ACCC were detected at two rs11465817 loci. 2. Rs10889677, the allele of rs11465817 locus and the frequency of genotype distribution had no significant difference between the case group and the control group (P 0.05). The frequency of genotype distribution of 3.rs10889677 locus in erosive type was significantly higher than that in control group (P 0.024). The risk of erosion of CC CA genotype individuals was 3.115 times that of AA genotype (95% CI: 1.135-8.548). Conclusion: 1. There were two loci of IL-23R gene in OLP population, rs10889677 rs11465817). SNP at rs11465817 site of 2.IL-23R gene had no significant correlation with susceptibility to OLP disease. The polymorphic variation of 3.rs10889677 locus may be related to the susceptibility and severity of OLP.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R781.5

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