游离氨基酸对变异链球菌生长及生物膜形成的影响

发布时间：2018-05-25 00:32

本文选题：变异链球菌 + 游离氨基酸　；参考：《牙体牙髓牙周病学杂志》2014年06期

【摘要】：目的:观察D-和L-游离氨基酸对变异链球菌生长及生物膜形成的影响。方法:将浓度为40 mmol/L的D-或L-半胱氨酸、酪氨酸、缬氨酸等20种游离氨基酸分别与变异链球菌UA159共同培养,分别于培养4、8、12、24 h各时间点对细菌进行活菌计数,以时间杀死(Tme-kill)法观察其对变异链球菌生长的影响;用结晶紫(Crystal violet,CV)染色法观察40 mmol/L游离氨基酸对变异链球菌生物膜形成的影响。结果:不同的氨基酸处理24 h后,D-半胱氨酸、D-精氨酸、L-赖氨酸可明显降低变异链球菌生存率(P0.05);D-和L-天冬氨酸、谷氨酸可抑制变异链球菌的生长(P0.05);D-苯丙氨酸、L-组氨酸、L-半胱氨酸处理组变异链球菌生存率呈现先下降后上升的趋势;D-和L-丝氨酸、酪氨酸、L-缬氨酸可延缓变异链球菌进入对数生长期;D-和L-谷氨酸、天冬氨酸、半胱氨酸、D-精氨酸、D-色氨酸可明显抑制变异链球菌生物膜的形成(P0.05)。结论:部分游离氨基酸对变异链球菌生长及生物膜的形成具有一定的抑制作用。
[Abstract]:Aim: to observe the effects of D-and L-free amino acids on the growth and biofilm formation of Streptococcus mutans. Methods: twenty free amino acids, such as D- or L-cysteine, tyrosine, valine and so on, were co-cultured with Streptococcus mutans UA159 at 40 mmol/L. The effects of Tme-kill on the growth of Streptococcus mutans and the effects of 40 mmol/L free amino acids on the biofilm formation of Streptococcus mutans were observed by Crystal violet C V staining. Results: after 24 h treatment with different amino acids, D-cysteine D-arginine L-lysine could significantly reduce the survival rate of Streptococcus mutans (P 0.05) and L-aspartic acid (L- aspartic acid). Glutamate inhibited the growth of Streptococcus mutans (P0.05). The survival rate of Streptococcus mutans decreased first and then increased in L-histidine L-cysteine treatment group. Tyrosine L-valine could delay the entry of Streptococcus mutans into logarithmic growth phase, such as D- and L-glutamic acid, aspartic acid, cysteine D-arginine, D-tryptophan, could significantly inhibit the formation of Streptococcus mutans biofilm. Conclusion: partial free amino acids can inhibit the growth and biofilm formation of Streptococcus mutans.
【作者单位】：中山大学光华口腔医学院·广东省口腔医学重点实验室;
【基金】：国家自然科学基金面上项目[2013(81371132)]
【分类号】：R781.1

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