层层静电自组装构建载药种植体的研究

发布时间：2018-05-28 06:38

本文选题：钛种植体 + 药物缓释　；参考：《华西口腔医学杂志》2014年06期

【摘要】：目的构建一种长效、靶向的携带促骨形成药物HU-308的药物缓释种植体,观察其体外缓释特性。方法采用层层静电自组装技术制备不同层数的肝素/壳聚糖涂层,以物理吸附的方式装载促骨形成药物HU-308,构建载药种植并进行体外释放实验。通过紫外可见光分光光度计测定药物浓度,分析不同涂层数载药种植体的加载效率及释放规律。用扫描电镜、原子力显微镜观察涂层表面形貌和结构的变化。结果成功地制备了载HU-308涂层种植体。体外释放实验表明,随着涂层层数的增加,载药量逐渐增加,但T20组略有下降。随着层数的增加,HU-308的释放速度随之减缓,缓释能力增强。扫描电镜、原子力显微镜结果表明种植体表面肝素/壳聚糖涂层逐渐形成。结论层层静电自组装技术成功构建载有HU-308的涂层种植体,可长期有效释放达30 d以上,这有望为临床上提高骨质疏松症患者种植体骨整合率提供一种新的可能。
[Abstract]:Aim to construct a long-acting, targeted drug sustained-release implant carrying bone forming drug HU-308 and observe its in vitro sustained release characteristics. Methods Heparin / chitosan coatings with different layers were prepared by layer electrostatic self-assembly technique. HU-308 was loaded with HU-308 by physical adsorption. The drug was implanted and released in vitro. The drug concentration was measured by UV-Vis spectrophotometer, and the loading efficiency and release rule of drug loaded implants with different coatings were analyzed. The surface morphology and structure of the coating were observed by scanning electron microscope and atomic force microscope. Results HU-308 coated implants were successfully prepared. In vitro release experiments showed that the drug loading increased with the increase of coating layer number, but decreased slightly in T20 group. With the increase of the number of layers, the release rate of HU-308 decreases and the slow release ability increases. The results of SEM and AFM showed that heparin / chitosan coating was gradually formed on the implant surface. Conclusion the layer by layer electrostatic self-assembly technique can successfully construct the coated implants containing HU-308, which can effectively release for more than 30 days, which is expected to provide a new possibility for improving the osseointegration rate of implants in patients with osteoporosis.
【作者单位】：芜湖市第一人民医院口腔科;广西医科大学口腔医院种植科;桂林理工大学材料科学与工程学院有色金属及材料加工新技术教育部重点实验室;
【基金】：广西医疗卫生重点科研课题基金资助项目(重2010083)
【分类号】：R783

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