实验性前导斜度改变的大鼠模型的建立及其颞下颌关节滑膜组织病理学观察

发布时间：2018-05-30 01:46

本文选题：前导斜度 + 前导缺失　；参考：《华西口腔医学杂志》2017年03期

【摘要】：目的建立稳定的前导斜度改变的大鼠模型,探索其对大鼠颞下颌关节(TMJ)滑膜的组织病理学影响。方法将32只大鼠随机分为4组,分别是对照组(C)、前导缺失组(T1)、前导斜度增大15°组(T2)、前导斜度增大30°组(T3)。在大鼠上前牙粘接舌侧增大15°、30°金属冠改变相应前导斜度。采用低速金刚砂轮打磨大鼠上下前牙,使下颌在各个方向运动时上下牙切缘不接触,造成前导缺失。3、7、14、28 d各处死2只。实验结束后取大鼠一侧TMJ作石蜡切片苏木精-伊红(HE)染色,通过组织病理学诊断鉴定大鼠TMJ滑膜的改变。结果 T1组体重短暂下降后缓慢增加(P0.05),T3组体重明显下降后略回升,但始终低于初始体重(P0.05),T3组滑膜衬里细胞增生,血管扩张明显(P0.05)。结论本研究所建立的前导斜度增大30°大鼠模型能较好地模拟临床上前导斜度过大造成的TMJ滑膜出现的组织病理变化过程。提示前导斜度增大越多,发生TMJ滑膜损伤的风险越大。而前导缺失对TMJ滑膜的影响有待进一步确定。
[Abstract]:Objective to establish a rat model with stable anterior gradient change and to explore its histopathological effects on TMJ synovium of temporomandibular joint (TMJ). Methods Thirty-two rats were randomly divided into 4 groups: control group (C), prednisone group (T _ (1), preconductance slope increased (15 掳) group (T _ (2), preconductance (30 掳) group (n = 30). The anterior canals were increased by 15 掳and 30 掳on the lingual side of the anterior teeth of the rat. The lower and lower anterior teeth of the rat were grinded with a low speed diamond wheel to make the upper and lower incisors of the mandible move in all directions without contact, resulting in the loss of the lead. The changes of TMJ synovium of rats were identified by histopathological diagnosis and paraffin sections of TMJ were stained with hematoxylin-eosin (HEH). Results the weight of T 1 group increased slowly after a short period of weight loss, and then increased slightly in group T 3, but it was always lower than that in group P 0.05 and T 3, but the proliferation of synovial lining cells and vasodilation were obvious in group T 3. Conclusion the rat model of 30 掳increase of preconductance can simulate the histopathological changes of TMJ synovium caused by excessive predilection in clinic. It suggested that the higher the leading slope, the greater the risk of TMJ synovial injury. The effect of leading loss on TMJ synovium needs to be further determined.
【作者单位】：西安交通大学口腔医院修复科;
【基金】：陕西省社会发展科技攻关基金(2012k16-10-03)~~
【分类号】：R-332;R782.6

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