牙龈卟啉单胞菌感染对大鼠血管平滑肌细胞细胞间黏附分子-1表达的影响

发布时间：2018-05-30 03:15

本文选题：牙龈卟啉单胞菌 + 血管平滑肌细胞　；参考：《华西口腔医学杂志》2014年02期

【摘要】：目的观察牙龈卟啉单胞菌ATCC 33277感染对大鼠血管平滑肌细胞(VSMC)细胞间黏附分子-1(ICAM-1)表达的影响。方法建立体外牙龈卟啉单胞菌感染大鼠VSMC模型,逆转录-聚合酶链反应(RT-PCR)检测ICAM-1基因的表达。结果牙龈卟啉单胞菌感染VSMC 8、16、24 h后,ICAM-1表达明显增多,与空白对照组比较,差异有统计学意义(P0.05)。感染16 h达高峰,感染8 h与感染16、24 h比较,差异有统计学意义(P0.05)。结论牙龈卟啉单胞菌感染可引起VSMC ICAM-1高表达,这提示牙周致病菌可能参与血管壁的炎症反应,在动脉粥样硬化的发生、发展中有重要的意义。
[Abstract]:Objective to investigate the effect of ATCC 33277 infection on the expression of intercellular adhesion molecule (ICAM-1) in rat vascular smooth muscle cells (VSMCs). Methods the VSMC model of rats infected with Porphyromonas gingivalis in vitro was established and the expression of ICAM-1 gene was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results the expression of ICAM-1 was significantly increased 24 h after infection with Porphyromonas gingivalis, and the difference was statistically significant compared with the control group (P 0.05). The infection reached the peak at 16 h, and the difference between 8 h and 1624 h was statistically significant (P 0.05). Conclusion Porphyromonas gingivalis infection can cause high expression of VSMC ICAM-1, which suggests that periodontal pathogenic bacteria may participate in the inflammation of vascular wall, and play an important role in the occurrence and development of atherosclerosis.
【作者单位】：哈尔滨医科大学附属第四医院口腔科;哈尔滨市口腔医院修复科;
【基金】：黑龙江省青年科学基金资助项目(QC2009C103) 黑龙江省教育厅科学技术研究基金资助项目(11511230)
【分类号】：R781.4

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