牙周治疗对伴动脉粥样硬化牙周炎大鼠血清IL-6及颈动脉MMP-2,9影响的研究

发布时间：2018-05-30 05:39

本文选题：牙周炎 + 动脉粥样硬化　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的通过检测伴动脉粥样硬化（atherosclerosis，As）的慢性牙周炎（chronicperiodontitis，CP）大鼠血清白细胞介素-6（Interleukin-6，IL-6）的浓度和颈动脉基质金属蛋白酶-2（Metalloproteinase-2，MMP-2）、基质金属蛋白酶-9（Metalloproteinase-9，MMP-9）的表达情况，研究辅以抗生素的牙周机械治疗对As病变的影响。方法 44只清洁级SD大鼠按随机数字表随机分为3大组，分别为A组（正常对照组）、B组（As组）、C组（CP+As组）。建模后，将C组再应用随机数字表分为4小组：C1自然进程组、C2单纯机械治疗组、C3机械治疗+派丽奥组和C4机械治疗+派丽奥+抗生素组。分别于建模后第1周、第3周、第5周、第7周和第9周酶联免疫吸附（ELISA）法检测血清中IL-6的浓度，，免疫组化法定性检测各组颈动脉血管壁组织的MMP-2、MMP-9并测量其灰度值。结果 1. MMP-2、MMP-9灰度值趋势基本一致，B、C组颈动脉的内、中膜均可见MMP-2、MMP-9高表达，其阳性染色深度均明显大于A组（P0.05）。B组可见大量泡沫细胞及平滑肌细胞，弹性纤维明显紊乱破坏；C1组（MMP-2，9灰度值分别为101.32.4、101.24.1）有动脉粥样硬化斑块形成，其阳性染色范围最大，染色深度明显强于B组（灰度值分别为126.42.0、124.82.8）（P0.05）；C2组血管壁厚薄不均，可见泡沫细胞， C3组血管壁增厚较轻，弹性纤维排列较整齐，C4组血管壁增厚，弹力纤维明显，可见泡沫细胞及SMC， C2、C3和C4组MMP-2、MMP-9免疫组化阳性染色范围及程度依次减弱，且均弱于C1组，灰度值差异有统计学意义（C2:134.53.1、136.73.2，C3:159.14.4、160.42.4， C4：170.32.3、168.82.2）（P0.05）。 2.血清结果显示，随着时间推移，B、C1组血清IL-6浓度一直呈升高趋势，均明显高于A组（P0.01）；C2、C3、C4组血清IL-6浓度均为先升后降，在建模后第5周达到峰值，其中C2组最高，从第4次取样时间点起，各组IL-6含量均显著低于C1组（P0.001），C3、C4组显著低于C2组（P0.01）。结论 1.对于伴有As的CP大鼠，牙周机械治疗可能会短期内增高机体炎症，而长期则可能降低血管炎症，减缓其As病变程度； 2.牙周机械治疗时辅以局部及全身抗炎药物后，颈动脉血管病变及全身炎症可以得到更好的改善； 3.若不及时控制慢性牙周炎症，则可能会加重As的病变程度,促进其动脉粥样硬化斑块的形成。
[Abstract]:Purpose The serum levels of interleukin-6 (IL-6) and the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in chronic periodontitis rats with atherosclerosis were determined. To study the effect of periodontal mechanical therapy with antibiotics on as lesions. Method Forty-four SD rats of clean grade were randomly divided into three groups: group A (normal control group, group B) and as group C (group C). After modeling, group C was randomly divided into 4 groups: C1 natural process group, C2 mechanical treatment group, C 3 mechanical therapy group and C4 mechanical treatment group. The concentration of IL-6 in serum was detected by Elisa at week 1, week 3, week 5, week 7 and week 9, respectively, and MMP-2 MMP-9 in carotid artery wall tissue was detected qualitatively by immunohistochemical method and its gray value was measured. Result 1. The intensity of MMP-2 and MMP-9 were similar in the carotid artery, and the high expression of MMP-2MMP 9 was found in the middle membrane. The positive staining depth of MMP-2MMP 9 was significantly higher than that in group A (P 0.05) and smooth muscle cells (SMC) were found in group B (P 0.05), and in group B (P 0.05), a large number of foam cells and smooth muscle cells were found. There were atherosclerotic plaques in group C _ 1, and the positive staining range was the largest, and the depth of staining was significantly stronger than that in group B (grayscale values were 126.42.0124.82.82.82.82.84.82.8respectively, the thickness and thickness of vascular wall were uneven in group C _ 2, P 0.05C _ 2, P _ (0.05) C _ 2, P _ (0.05) C _ (2), P _ (0.05) P _ (0.05) C _ 2). Foam cells were seen in C _ 3 group. The thickening of vascular wall and elastic fibers were observed in C _ 3 group and C _ 4 group. The range and degree of immunohistochemical staining of foam cell and SMC, C _ 2C _ 3 and C _ 4 group were decreased in turn. The grayscale value of C _ 2: 134.53.1136.73.2C _ 3: 159.14.4160.42.4 and C _ 4: 170.32. 3168.82.2 respectively were significantly different from that of C _ 2: 134.53.1136.73.2and C _ 4: 170.32. 33168.82.2 respectively. 2. The results showed that the serum IL-6 concentration in group C 1 was higher than that in group A (P 0.01) and then decreased, and reached the peak at the 5th week after modeling, and the highest in group C 2, and the concentration of IL-6 in group C 2 was higher than that in group C 2. From the time point of the fourth sampling, the content of IL-6 in each group was significantly lower than that in C _ 1 group (P 0.001) and C _ (3) C _ (3) C _ (4) group was significantly lower than that in C _ 2 group (P 0.01). Conclusion 1. For CP rats with as, periodontal mechanical therapy may increase inflammation in a short period of time, but in the long run it may decrease inflammation of blood vessels and slow down the degree of as lesion. 2. After periodontal mechanical treatment with local and systemic anti-inflammatory drugs, carotid artery lesions and systemic inflammation can be better improved. 3. If chronic periodontitis is not controlled in time, it may aggravate the pathological degree of as and promote the formation of atherosclerotic plaque.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.4;R543.5

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