大鼠萎缩腮腺中肌上皮细胞增殖状态的观察

发布时间：2018-06-05 03:45

本文选题：腮腺 + 萎缩　；参考：《临床与实验病理学杂志》2014年06期

【摘要】：目的采用免疫组化双染技术观察大鼠腮腺主导管结扎后萎缩腺体内肌上皮细胞(myoepithelial cell,MEC)的增殖变化规律。方法采用Wistar大鼠建立腮腺主导管结扎动物模型,结扎大鼠右侧腮腺主导管诱导腺体萎缩,HE染色观察正常腮腺及主导管结扎后1、3、5、7、14、21、30、60、90、120、150、180天萎缩性腮腺的组织学变化,采用免疫组化双重染色法定量分析MEC在腮腺萎缩不同时间点的数量、增殖率及分布情况。结果 HE染色示:主导管结扎后,腮腺腺泡细胞出现凋亡消失,大量导管样结构形成,纤维组织增生并伴有炎性细胞浸润。免疫组化染色示:导管结扎后第3天,MEC发生快速增殖,5天时增殖率达到顶峰,随后MEC增殖率降低,30~150天时MEC增殖率均维持较低水平,180天时难以观察到增殖的MEC且MEC细胞总数显著减少。结论结扎腮腺导管诱导腺体萎缩的过程中,MEC在萎缩早期表现出较强的增殖能力,5天时达到顶峰,随后增殖活力下降,30~150天MEC增殖活性均处较低水平,180天时已观察不到增殖的MEC。
[Abstract]:Objective to observe the proliferation of myoepithelial cells (MECs) of atrophy glandular myoepithelial cells in rat parotid gland after ligation of parotid gland by immunohistochemical double staining technique. Methods the animal model of parotid duct ligation was established in Wistar rats. The atrophy of the right parotid gland was induced by HE staining. The histological changes of the normal parotid gland and the atrophic parotid gland on the 1st day after the ligation of the parotid gland were observed. The number, proliferation rate and distribution of MEC at different time points of parotid atrophy were analyzed by immunohistochemical double staining. Results HE staining showed that after ligation of main duct, apoptosis of parotid acinar cells disappeared, a large number of catheter-like structures were formed, and fibrous tissue proliferated with inflammatory cell infiltration. Immunohistochemical staining showed that the proliferation rate of MEC reached its peak on the 3rd day after ligation. Then the proliferative rate of MEC decreased after 30 ~ 150 days, and the proliferative rate of MEC was maintained at a low level at 180 days. The proliferative MEC was difficult to be observed and the total number of MEC cells decreased significantly. Conclusion during the process of gland atrophy induced by ligation of parotid ducts, MECs showed a strong proliferative ability at the early stage of atrophy and reached the peak at 5 days, then the proliferative activity of MEC decreased at a low level of 180 days after 30 ~ 150 days of atrophy, and the proliferative activity of MECs was not observed at 180 days.
【作者单位】：滨州医学院附属医院口腔颌面外科;滨州医学院附属医院病理科;
【基金】：山东省医药卫生科技发展计划基金(2011HW-004)
【分类号】：R739.8

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