白花丹素增强术前诱导化疗PF方案对舌癌细胞的杀伤作用

发布时间：2018-06-09 07:13

本文选题：舌癌 + 白花丹素　；参考：《南昌大学》2014年硕士论文

【摘要】：目的：探讨白花丹素联合术前诱导化疗PF方案对舌癌细胞Tca8113的化疗增敏作用及其相关机制。方法： 1.舌癌Tca-8113细胞的细胞培养。 2.依次加入白花丹素、顺铂、5氟尿嘧啶，应用MTT法检测舌癌细胞抑制率，并计算最佳实验浓度IC10。 3. MTT法检测舌癌Tca8113细胞的细胞抑制率。 4.流式细胞术检测舌癌Tca-8113细胞的凋亡率。 5. Western Blot法检测舌癌Tca-8113细胞中PI3K、P-AKT蛋白表达水平的变化。 6. ELISA法检测舌癌Tca-8113细胞中PI3K、P-AKT蛋白表达水平的变化。结果： 1. MTT实验表明，白花丹素、顺铂、5氟尿嘧啶作用于舌癌Tca8113细胞24小时后，通过软件计算，其生长抑制浓度IC10依次为1.35μm/L、0.006μm/L、0.005μm/L。 2. MTT法检测结果表明，白花丹素联合PF方案较PF方案单独作用，其舌癌细胞的抑制率有显著增高（P0.05）。 3.流式细胞术检测结果表明，白花丹素联合PF方案较PF方案单独作用其舌癌细胞的凋亡率有显著增高（P0.05）。 4. Western Blot法检测结果显示，白花丹素联合PF方案较单独运用PF方案，其舌癌Tca8113细胞中PI3K、P-AKT蛋白表达水平显著降低（P0.05）。 5. ELISA法检测结果显示，白花丹素联合PF方案较单独运用PF方案，其舌癌细胞Tca8113中PI3K、P-AKT蛋白表达水平显著降低（P0.05）。结论：白花丹素具有化疗增敏作用，，增强了术前诱导化疗PF方案对舌癌Tca8113细胞的杀伤作用，抑制细胞中PI3K/AKT信号通路可能是其化疗增敏的主要机制。
[Abstract]:Objective: to investigate the chemosensitizing effect of Baihuadan combined with preoperation induced chemotherapy PF regimen on tongue cancer cell Tca8113. Methods: 1. Cell culture of Tca-8113 cells from tongue cancer. 2. The inhibitory rate of tongue cancer cells was detected by MTT assay, and the optimal concentration of IC10.3 was calculated by adding Bahuadanthin and cisplatin 5 fluorouracil in turn. MTT assay was used to detect the cell inhibitory rate of Tca8113 cells. The apoptosis rate of Tca-8113 cells was detected by flow cytometry. Western Blot assay was used to detect the expression of PI3KTP-AKT protein in Tca-8113 cells. Elisa method was used to detect the expression level of PI3KTP-AKT in Tca-8113 cells. Results: 1. MTT assay showed that the inhibitory concentration of IC10 was 1.35 渭 m / L ~ 0.006 渭 m / L ~ (0.005) 渭 m 路L ~ (-1) 路L ~ (-2) after 24 hours treatment of Tca8113 cell line with Baihuadan and cisplatin _ 5 fluorouracil. The IC10 concentration was 1.35 渭 m 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). MTT assay showed that compared with PF regimen alone, the inhibition rate of tongue cancer cells was significantly higher than that of PF regimen. The results of flow cytometry showed that compared with PF regimen alone, the apoptosis rate of tongue cancer cells was significantly higher than that of PF regimen alone. Western blot analysis showed that compared with PF alone, the expression of PI3KU P-AKT protein in Tca8113 cells decreased significantly. The results of Elisa showed that compared with PF regimen alone, the expression of PI3KU P-AKT protein in Tca8113 cells of tongue decreased significantly. Conclusion: Baihuadantin has the effect of chemosensitization, and the expression of PI3KUP-AKT protein in Tca8113 tongue cancer cells is significantly lower than that of PF regimen alone. The cytotoxicity of PF regimen to Tca8113 cells was enhanced, and the inhibition of PI3K / AKT signaling pathway may be the main mechanism of chemosensitization.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.86

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