牙周炎对小鼠脾脏巨噬细胞表型的影响

发布时间：2018-06-09 11:22

本文选题：牙周炎 + 脾脏　；参考：《实用医学杂志》2016年10期

【摘要】：目的:研究实验性牙周炎模型小鼠的脾脏巨噬细胞表型、分泌功能及相对数量变化,以探究牙周炎对脾脏巨噬细胞的影响。方法:22只22周龄小鼠随机分为真性结扎组(P10d组)和假性结扎对照组(C组),各11只牙周结扎10 d后处死。流式细胞术检测单核巨噬细胞及其M1、M2表型的比例。实时荧光定量PCR法检测脾脏中巨噬细胞相关抗炎型细胞因子IL-1β,抑炎型细胞因子IL-10促炎型mRNA相对表达水平的差异。结果:与C组相比,牙周炎组的M1型巨噬细胞占脾成熟巨噬细胞比例下降,M1/M2比例显著下降,M1型巨噬细胞炎症因子IL-1βmRNA表达水平下降。结论:慢性牙周炎能下调M1型巨噬细胞比例,使巨噬细胞表型M1/M2比例下降,促炎因子IL-1βmRNA表达水平下降。
[Abstract]:Aim: to study the phenotype, secretory function and relative quantity of splenic macrophages in experimental periodontitis model mice, and to explore the effect of periodontitis on splenic macrophages. Methods Twenty two 22-week-old mice were randomly divided into true ligation group (P10d group) and sham ligation control group (control group C). Each of 11 mice were killed after 10 days of periodontal ligation. The phenotypes of mononuclear macrophages and M _ 1 M _ 2 were detected by flow cytometry. The relative expression level of macrophage associated anti-inflammatory cytokine IL-1 尾 and anti-inflammatory cytokine IL-10 proinflammatory type mRNA was detected by real-time fluorescence quantitative PCR. Results: compared with group C, the ratio of M1 macrophages to spleen matured macrophages in periodontitis group was significantly lower than that in group C. The ratio of M 1 / M 2 to M 1 / M 2 in periodontitis group was significantly lower than that in group C, and the expression of IL-1 尾 mRNA in M 1 type macrophages was significantly decreased. Conclusion: chronic periodontitis can down-regulate the ratio of M1 macrophage, decrease the ratio of M 1 / M 2 and decrease the expression of IL-1 尾 mRNA.
【作者单位】：南方医科大学附属口腔医院广东省口腔医院;
【基金】：国家自然科学基金资助项目(编号:81371151)
【分类号】：R781.42

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