桥连上皮带在唇腭部发育过程中的作用及意义探讨

发布时间：2018-06-10 19:04

本文选题：上颌突 + 中鼻突　；参考：《实用口腔医学杂志》2016年03期

【摘要】：目的:研究上颌突、中鼻突之间的桥连上皮带在唇腭部发育过程中的作用。方法:将受精的白来航鸡蛋孵化到适当的H-H(hamburger and hamilton)阶段,分别进行:组织学切片、胚胎扫描电镜摄片、Brd U细胞增殖和TUNEL细胞凋亡实验。结果:鸡胚发育的H-H 24阶段,上颌突、中鼻突以及侧鼻突之间的上皮层相互靠近;H-H 26、27阶段,融合之前相邻突起之间的上皮细胞出现丝状伪足化,开始形成两两突起之间的桥连上皮带,之后桥连上皮带内细胞增殖减少,并出现了大量圆形凋亡细胞;H-H 28阶段:桥连上皮带在相邻突起融合过程中逐渐凋亡,相邻突起最终完全融合。结论:上颌突、中鼻突之间的桥连上皮带通过形成丝状伪足相互连接,桥连上皮带内细胞增殖减少、凋亡增加,这一系列变化在胚胎唇腭部的发育过程中起到了重要的作用。
[Abstract]:Objective: to study the role of bridging belt between maxillary process and middle nasal process in the development of lip and palate. Methods: the fertilized white Laihang eggs were incubated to the appropriate H-Hhamburger and Hamilton stage. The histological sections, the proliferation of Brd U cells and Tunel cell apoptosis were examined by scanning electron microscope (SEM). Results: in the H-H24 stage of chicken embryo development, the epithelium between maxillary process, middle nasal process and lateral nasal process was close to that of H-H2627, and the epithelial cells between adjacent processes appeared pseudopodization before fusion. The bridging belt between the two processes began to form, then the cell proliferation in the bridging belt decreased, and a large number of round apoptotic cells appeared in the H-H28 stage: the bridging belt gradually apoptosis in the process of fusion of adjacent processes. The adjacent protrusions are eventually fully integrated. Conclusion: the bridging belt between maxillary process and middle nasal process is connected with each other by forming filamentous pseudopodia. The cell proliferation and apoptosis increase in the bridging belt. This series of changes play an important role in the development of the embryonic lip and palate.
【作者单位】：广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室;首尔延世大学校齿科大学应用生命科学部;首尔延世大学校齿科大学口腔颚颜面外科;
【基金】：广东省科技计划项目(编号:2012B032000012) 广州市荔湾区科技计划项目(编号:20131215074-2) 广州医科大学归国人员基金(编号:2011C02)
【分类号】：R782

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