伊犁黑蜂蜂胶对口腔主要致龋细菌作用的体外实验

发布时间：2018-06-11 12:59

本文选题：蜂胶 + 致龋菌　；参考：《医学研究生学报》2014年12期

【摘要】：目的寻找天然防龋药物已成为国内外研究热点。文中研究伊犁黑蜂蜂胶对口腔常见致龋细菌毒力因子的作用,探讨其防龋效果。方法 1通过液体稀释法测定伊犁黑蜂蜂胶对口腔常见致龋菌的最小抑菌浓度(minimum inhibitory concentration,MIC);2以MIC以下的4个浓度配置成初始p H为7.0的含药培养基,以空白液体培养基作为对照组,加入菌液厌氧培养后测其p H值,并计算p H变化值(Δp H值)以检测不同浓度黑蜂蜂胶对测试菌产酸能力的影响;3蒽酮法测定MIC以下4个浓度(包括MIC浓度)的黑蜂蜂胶对变形链球菌、黏性放线菌产生胞外多糖的影响,空白液体培养基作为阳性对照组。结果 1伊犁黑蜂蜂胶对变形链球菌、远缘链球菌、嗜酸乳杆菌、血链球菌、黏性放线菌、内氏放线菌的MIC分别是0.78、0.39、1.56、0.39、0.2、0.2 mg/m L;最低杀菌浓度分别为1.56、0.78、3.125、0.78、0.39、0.39 mg/m L;2蜂胶可使各测试菌Δp H降低,蜂胶各组与阳性对照组相比差异均有统计学意义(P0.05);3在MIC浓度时,蜂胶可使变形链球菌、黏性放线菌合成胞外多糖的能力降低,蜂胶组与阳性对照组相比差异均有统计学意义(P0.05)。结论伊犁黑蜂蜂胶对口腔主要致龋细菌的生长、产酸、产糖均有不同程度的抑制作用,是具有一定防龋效果的天然药物。
[Abstract]:Objective to find natural anti-caries drugs has become a research hotspot at home and abroad. In this paper, the effect of Propolis on the virulence factor of common dental caries bacteria was studied, and the effect of propolis on dental caries was discussed. Methods 1 the minimum inhibitory concentration of propolis against common cariogenic bacteria in oral cavity was determined by liquid dilution method. Four concentrations below MIC were used as the initial pH 7.0 medium, and the blank liquid medium was used as the control group. The pH value of bacteria was measured after anaerobic culture. The pH value (螖 pH value) was calculated to detect the effect of different concentrations of black bee propolis on the acid-producing ability of test bacteria. The four concentrations (including MIC concentration) of black bee propolis against Streptococcus mutans were determined by anthrone method. The effect of actinomycetes on the production of extracellular polysaccharides, blank liquid medium as a positive control group. Results 1 Propolis of Ili wasp against Streptococcus mutans, Streptococcus distalis, Lactobacillus acidophilus, Streptococcus sanguis, actinomycetes. The mics of Actinomyces Nei were 0.78 ~ 0.39 ~ (0.39) ~ (0.39) ~ 0.39 ~ 0.22 mg/m / L, respectively, and the minimum bactericidal concentration of propolis was 1.56 ~ 0.78 ~ (3.125) ~ 0.39 mg/m / L ~ (2) propolis, respectively. Propolis could make Streptococcus mutans (Streptococcus mutans) lower than that of the positive control group (P _ (0.05) ~ (3) in the concentration of propolis (P _ (0.05), P _ (0.05) (P _ (0.05) and P _ (0.05) 渭 mol 路L ~ (-1), respectively. The ability of actinomycetes to synthesize extracellular polysaccharides decreased, and the difference between propolis group and positive control group was statistically significant (P 0.05). ConclusionThe propolis has different inhibitory effects on the growth, acid production and sugar production of the main dental caries bacteria, and is a natural drug with a certain anti-caries effect.
【作者单位】：新疆医科大学第一附属医院牙体牙髓科/新疆维吾尔自治区口腔医学研究所;新疆医科大学维吾尔医药学院;
【基金】：新疆维吾尔自治区地方病分子生物学实验室开放课题(XJDX0208-2011-06)
【分类号】：R780.2

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