体外培养的人牙胚上皮细胞的成釉分化

发布时间：2018-06-16 02:22

本文选题：人牙胚上皮细胞 + 成釉分化　；参考：《中国生物化学与分子生物学报》2014年01期

【摘要】：人表皮干细胞可以作为牙齿再生中上皮源性的种子细胞,但是其成釉分化的效率低下.本研究分离培养了人牙胚上皮细胞,利用E13.5的小鼠牙间充质与其重组,构建重组牙胚,对其成釉分化的潜能和机制进行研究.研究结果发现,体外培养的P1代人牙胚上皮细胞成釉率高达50%.随着传代次数的增加,成釉率明显下降.通过对牙上皮发育分化相关基因的表达检测和分析表明,重组牙胚成牙分化能力和成釉潜能的下降与牙上皮发育相关基因的表达状态密切相关.特别是FGF8表达水平的下调以及PITX2不同亚型在人牙胚细胞中表达量的不均衡,可能是导致人牙胚细胞成釉潜能下降并丧失的主要原因.本研究结果为理解牙齿再生过程中上皮源性的种子细胞的成釉机制提供了新的实验数据,对进一步提高表皮干细胞在牙齿再生过程中的成釉率有指导意义.
[Abstract]:Human epidermal stem cells can be used as epithelial seed cells in tooth regeneration, but their enamel differentiation efficiency is low. In this study, human tooth germ epithelial cells were isolated and cultured, and the potential and mechanism of enamel differentiation of E13.5 mouse tooth mesenchymal cells were studied. The results showed that the enamel forming rate of P1 passage human tooth germ epithelial cells was as high as 50%. With the increase of passage times, the glaze formation rate decreased obviously. The expression and analysis of the genes related to the development and differentiation of tooth epithelium showed that the decline of tooth differentiation ability and enamel potential of the recombinant tooth germ was closely related to the expression of the genes related to the development of tooth epithelium. Especially the down-regulation of FGF8 expression and the imbalance of the expression of PITX2 subtypes in human tooth germ cells may be the main reasons for the decrease and loss of enamel potential of human tooth germ cells. The results of this study provide new experimental data for understanding the mechanism of enamel formation of epithelial-derived seed cells during tooth regeneration, and provide guidance for further improving the enamel forming rate of epidermal stem cells during tooth regeneration.
【作者单位】：福建省发育与神经生物学重点实验室福建师范大学生命科学学院;
【基金】：国家自然科学基金项目(No.81100730) 福建省自然科学基金(No.2012J01119) 福建省教育厅科技项目(No.JA11048)~~
【分类号】：R780.2

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