血管内皮细胞对牙周组织细胞迁移的影响

发布时间：2018-06-16 08:33

本文选题：牙周再生 + 血管内皮细胞　；参考：《上海口腔医学》2014年02期

【摘要】：目的:通过血管内皮细胞与牙周组织细胞的复合培养模拟牙周组织再生环境,研究血管内皮细胞对牙周组织细胞迁移的影响,为明确血管内皮细胞在牙周组织再生过程中的作用奠定基础。方法:应用原代培养的血管内皮细胞,在Transwell嵌套中实现与人牙周膜成纤维细胞、牙龈成纤维细胞间的复合培养。分别以Transwell嵌套滤膜下腔面24 h的细胞数量检测垂直迁移能力,以划痕实验后0、8、16和24 h计数划痕区域的细胞数量检测水平迁移能力.以玻片试验及计算机辅助图像处理软件计算实验第1、4和7天细胞覆盖创面的面积检测覆盖创面能力.并均与单独培养的2种牙周组织细胞作为对照。采用SPSS13.0软件包对实验结果进行统计学分析。结果:血管内皮细胞存在时,2种牙周细胞在垂直和水平方向的迁移量均显著高于单独培养组(P0.01),且牙周膜成纤维细胞垂直向的迁移量显著高于牙龈成纤维细胞(P0.01),但其水平迁移量在实验24 h时则显著低于牙龈成纤维细胞(P0.01):在血管内皮细胞共存条件下,2种牙周细胞创面覆盖能力在实验第7天高于对照组,且牙周膜成纤维细胞的创面覆盖能力的增加显著高于牙龈成纤维细胞(P0.01)。结论:血管内皮细胞对牙周膜、牙龈成纤维细胞的迁移和创面覆盖有明显促进作用,且血管内皮细胞对牙周膜成纤维细胞的垂直向迁移和创面覆盖能力的促进作用更为明显。
[Abstract]:Objective: to study the effects of vascular endothelial cells (VEC) on periodontal tissue migration by co-culture of vascular endothelial cells (VEC) and periodontal tissue cells (PDCs). It lays a foundation for understanding the role of vascular endothelial cells in periodontal tissue regeneration. Methods: the primary cultured vascular endothelial cells were mixed with human periodontal ligament fibroblasts and gingival fibroblasts in Transwell nesting. The vertical migration ability was detected by the number of cells in the surface of the sublumen of the transwell nested filter for 24 h, and the horizontal migration ability was measured by counting the number of cells in the scratch area at 0 ~ 816 h and 24 h after scratch test. The ability of covering wounds was measured with glass slide test and computer aided image processing software on the 4th and 7th day of experiment. All of them were compared with two kinds of periodontal tissue cells cultured alone. The experimental results were analyzed by SPSS 13.0 software package. Results: in the presence of vascular endothelial cells, the vertical and horizontal migration of periodontal cells was significantly higher than that of P0.01in the isolated culture group, and the vertical migration of periodontal ligament fibroblasts was significantly higher than that of the gingival fibroblasts. The horizontal migration was significantly lower than that of the gingival fibroblasts at 24 h. The wound covering ability of the two periodontal cells was higher than that of the control group on the 7th day under the condition of co-existence of vascular endothelial cells. The wound coverage of periodontal ligament fibroblasts was significantly higher than that of gingival fibroblasts. Conclusion: vascular endothelial cells can significantly promote the migration of periodontal ligament and gingival fibroblasts and the wound coverage, and the vascular endothelial cells can promote the vertical migration of periodontal ligament fibroblasts and the ability of wound coverage.
【作者单位】：上海交通大学医学院附属第九人民医院·口腔医学院牙周病科上海市口腔医学重点实验室;
【分类号】：R781.4

【参考文献】