干细胞因子在人慢性根尖周炎组织中表达的研究

发布时间：2018-06-16 10:06

本文选题：慢性根尖周炎 + 干细胞因子　；参考：《口腔医学研究》2017年02期

【摘要】：目的:观察干细胞因子(stem cell factor,SCF)在人慢性根尖周炎病变组织中成纤维细胞(fibroblasts,FBs)、内皮细胞(endothelial cells,ECs)及巨噬细胞(macrophages,m錙)上的表达,探讨SCF阳性的FBs、ECs及m錙在人慢性根尖周炎发病机制中的作用。方法:本实验共收集50例根尖周组织标本,其中根尖周肉芽肿组15例,根尖周囊肿组15例,正常对照组20例。组织标本经福尔马林液固定,石蜡包埋、制作连续切片。HE染色,光学显微镜下观察各组标本的组织学变化;经免疫荧光双染色(double immunofluorescence,DIF),在荧光显微镜下观察SCF在根尖周组织FBs、ECs及m錙上表达的情况。结果:DIF结果显示,两组慢性根尖周炎组织中的CD334-SCF双阳性FBs、CD31-SCF双阳性ECs及CD14-SCF双阳性m錙密度较正常对照组显著增多(P0.01);根尖周囊肿组与根尖周肉芽肿组的CD334-SCF双阳性FBs及CD31-SCF双阳性ECs密度无显著性差异;根尖周肉芽肿组CD14-SCF双阳性m錙密度明显高于根尖周囊肿组(P0.01)。结论:结果提示CD334-SCF双阳性FBs、CD31-SCF双阳性ECs及CD14-SCF双阳性m錙与慢性根尖周炎的发生、发展及致病机制有关。
[Abstract]:Objective: to observe the expression of stem cell factor (SCF) in fibroblast fibroblasts (FBs1), endothelial cells (ECs) and macrophage (macrophagesm) of human chronic periapical periodontitis. To investigate the role of SCF-positive FBsSECs and mzil in the pathogenesis of chronic periapical periodontitis. Methods: a total of 50 periapical tissue specimens were collected, including 15 periapical granuloma group, 15 periapical cysts group and 20 normal controls. The tissue samples were fixed with formalin solution and embedded in paraffin wax. The histological changes of each group were observed under optical microscope. The expression of SCF in the periapical tissue of FBsSU ECs and MZL was observed under fluorescence microscope by double immunofluorescence staining. The result is: DIF, it turns out, The density of CD334-SCF and CD14-SCF double positive ECs and CD14-SCF in chronic periapical periodontitis was significantly higher than that in control group (P 0.01), and there was no significant difference in the density of CD334-SCF double positive FBs and CD31-SCF double positive ECs between periapical cyst group and periapical granuloma group, and there was no significant difference in the density of CD334-SCF double positive ECs and CD31-SCF double positive ECs between periapical cyst group and periapical granuloma group. The double positive density of CD14-SCF in periapical granuloma group was significantly higher than that in periapical cyst group (P 0.01). Conclusion: the results suggest that the double positive cells of CD334-SCF, CD31-SCF and CD14-SCF are related to the occurrence, development and pathogenesis of chronic periapical periodontitis.
【作者单位】：暨南大学医学院口腔医学系;
【基金】：广东省社会发展领域科技计划项资助项目(编号:2013B021800043;2014A020212212)
【分类号】：R781.341

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