Pyk2和p-AKT在舌癌组织中的表达及临床意义

发布时间：2018-06-17 02:33

本文选题：Pyk2 + p-AKT　；参考：《青岛大学》2017年硕士论文

【摘要】：目的:探讨富含脯氨酸的酪氨酸激酶2(Pyk2)和磷酸化的丝氨酸/苏氨酸蛋白激酶(p-AKT)在舌鳞状细胞癌组织(TSCC)及其癌旁非肿瘤组织中的表达差异及临床意义。方法:收集青岛大学附属烟台毓璜顶医院2012年1月至2016年5月手术切除的45例舌癌组织标本及其配对癌旁非肿瘤组织30例作为研究对象。采用免疫组织化学法检测Pyk2、p-AKT蛋白的表达情况。应用SPSS19.0统计学软件分析二者蛋白表达水平与舌癌患者性别、年龄、肿瘤直径、病理分化程度、临床分期、淋巴结转移等临床病理学参数之间的关系以及Pyk2、p-AKT蛋白的相关性。结果:(1)Pyk2和p-AKT蛋白在舌癌组织中阳性表达率高于癌旁非肿瘤组织。Pyk2蛋白在舌癌组织、癌旁非肿瘤组织中的阳性表达率分别为57.78%、6.67%(χ2=17.937,P=0.000),并且p-AKT阳性表达率分别为55.56%、16.67%(χ2=9.780,P=0.002),差异均较明显,具有统计学意义。(2)Pyk2蛋白的表达在病理分化程度中,高分化者阳性表达率为46.87%,中、低分化者阳性表达率约为84.62%(P0.05)。TNM分期中,Ⅰ-Ⅱ期阳性表达率约为41.94%,Ⅲ-Ⅳ期阳性表达率为92.85%(P0.05)。有淋巴结转移组阳性表达率约为91.67%,无淋巴结转移组阳性表达率约为45.45%(P0.05)。Pyk2蛋白的表达与病理分化程度、临床分期及区域淋巴结转移有关,而与性别、年龄、肿瘤直径之间无统计学意义。(3)p-AKT蛋白的表达在病理分化程度中,高分化者阳性表达率约为43.75%,中、低分化者阳性表达率为84.62%,差异具有统计学意义(P0.05)。p-AKT蛋白的表达只与病理分化程度有关,与性别、年龄、肿瘤直径、临床分期及区域淋巴结转移无关。(4)45例舌癌组织中Pyk2阳性表达26例,其中p-AKT共同表达阳性16例。(5)30例癌旁非肿瘤组织中,Pyk2、p-AKT蛋白同时表达阴性13例。经Spearman秩相关分析,二者呈正相关(P=0.005,γs=0.412)。结论:在舌癌组织中Pyk2、p-AKT蛋白呈高表达,而在癌旁非肿瘤组织中呈低表达甚至无表达。Pyk2蛋白的表达与病理分化程度、临床分期及区域淋巴结转移有关,而p-AKT蛋白的表达只与病理分化程度有关。p-AKT代表了P13K/AKT信号通路的的激活状态,Pyk2作为该通路的上游分子,二者之间表现出正相关,提示Pyk2可能通过参与P13K/AKT信号转导通路引起或促进舌癌的发生、发展,联合检测二者有望成为TSCC恶性程度的指标。并可能作为新的治疗靶点应用于肿瘤的生物靶向治疗,为舌癌的早期筛查、临床诊断、预后评价与监测提供新标识。
[Abstract]:Objective: To investigate the difference and clinical significance of the expression of proline rich tyrosine kinase 2 (Pyk2) and phosphorylated serine / threonine protein kinase (p-AKT) in tongue squamous cell carcinoma (TSCC) and adjacent nontumor tissues. Methods: 45 cases of surgical excision from January 2012 to May 2016 of the affiliated Yantai Yuhuangding Hospital of Qiingdao University were collected. 30 cases of tongue cancer tissue and 30 cases of non cancer tissue adjacent to cancer were used as research subjects. Immunohistochemical method was used to detect the expression of Pyk2 and p-AKT protein. The expression level of two proteins was analyzed by SPSS19.0 statistics software and the sex, age, tumor diameter, pathological differentiation, clinical staging, lymph node metastasis, and so on. The relationship between the pathological parameters and the correlation of Pyk2 and p-AKT protein. Results: (1) the positive expression rate of Pyk2 and p-AKT protein in tongue cancer tissues was higher than that of.Pyk2 protein in the nontumor tissues of the noncancerous tissues, and the positive expression rates in non tumor tissues were 57.78%, 6.67% (2=17.937, P=0.000), respectively, and the positive expression rate of p-AKT was respectively 55.56%, 16.67% (x 2=9.780, P=0.002), the difference was significant. (2) the expression of Pyk2 protein was in the degree of pathological differentiation, the positive expression rate of the high differentiation was 46.87%, and the positive expression rate of the low differentiation was about 84.62% (P0.05).TNM stage, the positive expression rate of I to II was about 41.94%, and the positive expression rate of stage III to IV was 92.85% (P0.0 5) the positive expression rate in the lymph node metastasis group was about 91.67%, the positive expression rate of the non lymph node metastasis group was about 45.45% (P0.05), the expression of.Pyk2 protein was related to the degree of pathological differentiation, the clinical stage and the regional lymph node metastasis, but there was no statistical significance between the sex, age and tumor diameter. (3) the expression of p-AKT protein was in the degree of pathological differentiation, The positive expression rate of the high differentiation was about 43.75%, and the positive expression rate of the low differentiation was 84.62%. The difference was statistically significant (P0.05) the expression of.P-AKT protein was only related to the degree of pathological differentiation, and was not related to sex, age, tumor diameter, clinical stage and regional lymph node metastasis. (4) 26 cases of positive expression of Pyk2 in 45 cases of tongue cancer, of which p-AKT was common. 16 cases of the same expression were positive. (5) 30 cases of non tumor tissues adjacent to cancer, Pyk2 and p-AKT protein were negative in 13 cases. The two was positive correlation (P=0.005, gamma s=0.412) by Spearman rank correlation analysis. Conclusion: Pyk2, p-AKT protein is highly expressed in the tongue cancer tissue, and the expression of low expression or no expression of.Pyk2 protein in the nontumor tissue adjacent to the carcinoma and even no expression of.Pyk2 protein The degree of differentiation, clinical staging and regional lymph node metastasis, and the expression of p-AKT protein only associated with the degree of pathological differentiation.P-AKT represents the activation state of the P13K/AKT signaling pathway. Pyk2 is the upstream molecule of the pathway, and there is a positive correlation between the two, suggesting that Pyk2 may be caused or promoted by the participation of the P13K/AKT signal transduction pathway. The occurrence, development and joint detection of tongue cancer are expected to be an indicator of the malignancy of TSCC, and may be used as a new target for the biological target therapy of tumor. It provides a new identification for the early screening, clinical diagnosis, prognosis evaluation and monitoring of tongue cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.86

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