增殖细胞核抗原和P53在萎缩性腮腺再生中的表达及意义

发布时间：2018-06-19 01:56

本文选题：腮腺 + 萎缩　；参考：《华西口腔医学杂志》2017年06期

【摘要】：目的从基因和蛋白水平研究增殖细胞核抗原(PCNA)和P53在萎缩性腮腺再生过程中的表达及意义。方法将102只Wistar大鼠分为实验组和对照组,结扎实验组大鼠腮腺主导管,分别于结扎7 d(A组)、14 d(B组)后实现腮腺导管再通,并于再通后第0、3、5、7、10、14、21、28天处死大鼠获取新鲜腮腺组织标本。采用苏木精-伊红(HE)染色法观察腮腺组织形态学变化,实时荧光定量聚合酶链式反应和Western blot法检测两组腮腺组织中PCNA和P53的表达。结果两实验组在腮腺导管结扎第7、14天,腺泡凋亡,导管细胞增殖,同组P53相对表达量高于PCNA;随着再通时间延长,腺泡细胞逐渐增多,A组在导管再通后第3天、B组在导管再通后第5天,PCNA和P53相对表达量达到峰值,与对照组比较,差异有统计学意义(P0.01);其后PCNA和P53相对表达量逐渐减少,A组于再通后第21天、B组于再通后第28天接近对照组。结论腮腺导管结扎后,P53相对表达量增多,诱导腮腺腺泡凋亡;导管再通后,PCNA相对表达量逐渐增多,达峰值后减少,可促进腺泡增殖。
[Abstract]:Objective to study the expression and significance of proliferating cell nuclear antigen (PCNA) and p53 in atrophic parotid gland regeneration from gene and protein levels. Methods 102 Wistar rats were divided into experimental group and control group. The parotid duct of experimental group was ligated. After 7 days of ligation, the parotid duct was recanalized in group A (n = 14). The histomorphologic changes of parotid gland were observed by hematoxylin and eosin (HEH) staining. The expression of PCNA and p53 in parotid gland was detected by real-time fluorescence quantitative polymerase chain reaction and Western blot. Results on the 714th day after ligation of parotid duct, apoptosis of acinus and proliferation of ductal cells were observed in the two experimental groups. The relative expression of p53 in the same group was higher than that in PCNA. The relative expression of PCNA and p53 in group B reached the peak on the 5th day after the recanalization of the ductus, compared with the control group. The relative expression of PCNA and p53 decreased gradually in group A on the 21st day after recanalization, and close to the control group on the 28th day after recanalization. Conclusion the relative expression of p53 was increased after ligation of parotid duct, which induced apoptosis of parotid gland acinar, and PCNA expression increased gradually after catheter recanalization, decreased after peak value, and promoted the proliferation of acinus.
【作者单位】：滨州医学院附属医院口腔颌面外科;滨州医学院附属医院药学部;滨州医学院附属医院病理科;滨州医学院附属医院口腔内科;
【基金】：山东省医药卫生科技发展计划(2011HW004,2015WS0487)~~
【分类号】：R781.7

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