低氧预处理及AMPK过表达对过氧化氢抑制牙髓细胞增殖影响的研究

发布时间：2018-06-20 06:19

  本文选题：低氧预处理 + 腺苷酸单磷酸激活蛋白激酶(AMPK)　； 参考：《牙体牙髓牙周病学杂志》2014年07期

【摘要】：目的:观察低氧预处理以及腺苷酸单磷酸激活蛋白激酶(AMPK)过表达对过氧化氢(H2O2)抑制牙髓细胞增殖的影响。方法:牙髓细胞分别在常氧(950 mL/L O2)和低氧(10 mL/L O2)环境下培养12 h后,用qRT-PCR检测细胞中AMPK mRNA的表达水平;分别取常氧和低氧培养12 h的牙髓细胞与0.1 mmol/L H2O2共培养4 h后,常规条件下继续培养4 d,用MTT法检测细胞的增殖能力;分别用AMPK过表达质粒和特异性小RNA干扰(siRNA)片段转染牙髓细胞,与0.1 mmol/L H2O2共培养4 h,然后常规条件下继续培养4 d,用MTT法检测细胞的增殖能力。结果:低氧预处理能显著上调牙髓细胞中AMPK mRNA的表达水平,并能减轻H2O2对细胞增殖的抑制作用(P0.05);AMPK过表达细胞经过H2O2处理后,增殖能力明显高于正常细胞+H2O2组和siRNA+H2O2组(P0.05),而siRNA干扰的细胞经H2O2处理后其增殖能力则较其他各组降低(P0.05)。结论:低氧预处理和AMPK基因的上调可使牙髓细胞获得对H2O2氧化的抗性,从而减轻H2O2对细胞增殖的抑制作用。
[Abstract]:Aim: to investigate the effects of hypoxia preconditioning and overexpression of adenylate monophosphate-activated protein kinase (AMPK) on the proliferation of dental pulp cells inhibited by H _ 2O _ 2 H _ 2O _ 2. Methods: dental pulp cells were cultured in normoxic (950 mL / L) and hypoxia (10 mL / L) for 12 h. The expression of AMPK mRNA in dental pulp cells was detected by qRT-PCR and co-cultured with 0.1 mmol / L H _ 2O _ 2 for 4 h in normoxic and hypoxic culture for 12 h. The proliferation of dental pulp cells was detected by MTT assay and transfected into dental pulp cells by AMPK overexpression plasmids and specific small RNA interference siRNAs. The cells were co-cultured with 0.1 mmol / L H _ 2O _ 2 for 4 h, then cultured for 4 days under conventional conditions. The proliferation of the cells was measured by MTT assay. Results: hypoxia pretreatment could significantly up-regulate the expression of AMPK mRNA in dental pulp cells and reduce the inhibitory effect of H _ 2O _ 2 on cell proliferation. The proliferative ability of siRNA interference cells was significantly higher than that of normal cells treated with H 2O 2 and siRNA H 2O 2 groups, but the proliferation ability of siRNA interference cells treated with H 2O 2 was lower than that of other groups. Conclusion: hypoxia preconditioning and up-regulation of AMPK gene can make dental pulp cells become resistant to H2O2 oxidation, thus reducing the inhibitory effect of H2O2 on cell proliferation.
【作者单位】： 安康市中心医院口腔科;神木县医院口腔科;
【分类号】：R780.2
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本文编号：2043285