参与不同牙本质粘接界面退变过程中基质金属蛋白酶种类的研究

发布时间：2018-06-20 22:22

本文选题：牙本质 + 粘接　；参考：《华西口腔医学杂志》2014年04期

【摘要】：目的研究参与两种粘接剂形成的牙本质粘接界面退变过程中基质金属蛋白酶(MMPs)的种类。方法制备Single Bond 2和Clearfi l S3 Bond微剪切粘接试件,无菌人工唾液中分别浸泡0、6个月测试粘接强度,场发射扫描电镜(FE-SEM)观察界面断裂模式。制备4 mm×3 mm×1 mm牙本质片,分为对照组、Single Bond 2组和Clearfi l S3Bond组,无菌人工唾液浸泡0、6个月后,液相芯片技术测定各组MMP-1、-2、-3、-8、-9含量。结果老化6个月后,各组粘接强度显著下降。与对照组相比,老化过程中不同粘接剂组MMP-1、-3含量无明显变化,Single Bond 2组MMP-2、-8、-9含量显著降低,Clearfi l S3 Bond组MMP-8、-9含量显著降低。结论老化6个月后两种粘接试件均发生粘接界面退变,牙本质源MMP-2、-8、-9的含量显著降低,提示这些MMPs可能参与了牙本质粘接界面的退变。
[Abstract]:Objective to study the types of matrix metalloproteinase (MMPs) involved in dentin bonding interface degeneration. Methods single Bond 2 and Clearfi l S3 Bond microshear bonding specimens were prepared. The bonding strength was measured by immersion in sterile artificial saliva for 0 months and the fracture mode of interface was observed by field emission scanning electron microscope (SEM). 4 mm 脳 3 mm 脳 1 mm dentin films were prepared and divided into control group (single Bond 2) and Clearfi l S3Bond group. After 6 months of immersion in aseptic artificial saliva, the content of MMP-1 / 2 +-3 + -8 + -8 / -9 was determined by liquid chip technique. Results after 6 months of aging, the adhesive strength of each group decreased significantly. Compared with the control group, there was no significant change in the content of MMP-1 +-3 in different adhesive groups during aging. The content of MMP-2-8 + -9 in single Bond 2 group was significantly lower than that in the control group. The content of MMP-8-9 in Clearfi l S3 group was significantly lower than that in Clearfi l S3 group. Conclusion after 6 months of aging, the adhesion interface degeneration occurred in both specimens, and the content of MMP-2 + -8 + -9 decreased significantly, suggesting that these MMPs may be involved in the degeneration of dentin adhesion interface.
【作者单位】：西安医学院口腔医学系口腔修复学教研室;第四军医大学口腔医院修复科;
【基金】：国家自然科学基金资助项目(51373198,81100772,81-130078)
【分类号】：R783.2

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