miR-203在舌癌组织中的表达及其对Tca8113细胞活力和侵袭的影响

发布时间：2018-06-23 08:42

本文选题：miR- + 舌癌　；参考：《中国病理生理杂志》2016年10期

【摘要】：目的:研究舌癌组织中miR-203的表达,分析它与临床病理学参数之间的相关性,并探讨其与人舌癌Tca8113细胞活力及侵袭能力的关系。方法:收集28例舌癌手术标本,每例标本分别取癌灶及远端5 cm处正常黏膜组织(即远癌正常组织)2个部分。实时定量PCR检测舌癌患者肿瘤组织中miR-203的相对表达水平,分析其与临床病理特征之间的关系;脂质体2000介导miR-203 mimic和无关序列对照转染Tca8113细胞株,并通过CCK-8实验和Transwell小室侵袭实验检测上调miR-203对Tca8113细胞活力及侵袭能力的影响。结果:28例舌癌组织中miR-203表达水平与癌旁正常组织相比显著降低(P0.05),miR-203的表达与肿瘤TNM分期及淋巴结转移存在相关性(P0.05),而与患者的年龄和性别无关。Tca8113细胞转染miR-203 mimics上调miR-203表达可显著抑制Tca8113细胞株的活力及侵袭能力,与对照组比较差异有统计学显著性(P0.05)。结论:上调miR-203表达能有效抑制舌癌细胞的生长和侵袭,miR-203可能成为舌癌基因治疗的靶点。
[Abstract]:Objective: to study the expression of miR-203 in human tongue carcinoma, to analyze the correlation between miR-203 and clinicopathological parameters, and to explore the relationship between miR-203 expression and the viability and invasiveness of Tca8113 cells. Methods: a total of 28 cases of tongue cancer were collected. Each specimen was divided into two parts: the normal mucosa at the distal end of 5 cm and the normal tissue of the distal carcinoma. The relative expression of miR-203 was detected by real-time quantitative PCR, and the relationship between miR-203 and clinicopathological features was analyzed. Liposome 2000 mediated miR-203 mimic and unrelated sequence control were transfected into Tca8113 cell line. The effect of upregulation of miR-203 on the viability and invasiveness of Tca8113 cells was detected by CCK-8 and Transwell chamber invasion assay. Results the expression level of miR-203 in 28 cases of tongue cancer was significantly lower than that in adjacent normal tissues (P0.05). The expression of miR-203 was correlated with tumor stage and lymph node metastasis (P0.05), but not related to age and sex of patients. Tca8113 cells transfected miR-203 mimics was up-regulated. The expression of miR-203 significantly inhibited the viability and invasion of Tca8113 cells. Compared with the control group, the difference was statistically significant (P0.05). Conclusion: upregulation of miR-203 expression can effectively inhibit the growth and invasion of tongue cancer cells. MiR-203 may be the target of tongue oncogene therapy.
【作者单位】：河南大学医学院病理生理学教研室;河南大学淮河临床学院口腔科;河南大学医学院免疫学教研室;
【基金】：开封市科技发展计划项目(No.1503112) 河南大学科研基金重点项目(No.2010ZRZD02)
【分类号】：R739.86

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