CD147和CypA在人慢性根尖周炎中的表达及临床意义

发布时间：2018-06-27 13:22

本文选题：细胞外基质金属蛋白酶诱导因子 + 亲环素A　；参考：《安徽医科大学》2016年硕士论文

【摘要】：目的检测细胞外基质金属蛋白酶诱导因子(Extracellular Matrix Metalloproteinase Inducer,CD147,EMMPRIN)和亲环素A(Cyclophilin A,Cyp A)在人根尖肉芽肿和根尖囊肿中的表达,探讨CD147和Cyp A在人慢性根尖周炎发生发展中的作用。方法从2014年12月至2015年5月,在安徽医科大学附属口腔医院口腔颌面外科收集经拔牙或根尖手术切除,并经病理鉴定为根尖肉芽肿(35例)的和根尖囊肿(30例)的组织作为实验组,同时收集行牙槽骨修整术凿下的或埋伏阻生智齿拔除时8例健康牙槽骨作为正常对照组。根据CBCT图像记录平均根尖周透射影直径大小作为根尖周病变大小。运用免疫组织化学法检测所有样本中CD147和Cyp A蛋白的表达,根据病变类型(根尖肉芽肿、根尖囊肿)和根尖周病变大小,分析CD147和Cyp A的蛋白表达水平。结果免疫组织化学的连续切片显示:CD147和Cyp A主要表达于炎性和上皮区域,主要为淋巴细胞、上皮细胞、巨噬细胞、成纤维细胞等。CD147和Cyp A存在于所有根尖周病变组织中。正常对照组中少见CD147、Cyp A表达,阴性对照组中未见CD147、Cyp A阳性表达。CD147、Cyp A在根尖肉芽肿和根尖囊肿中的蛋白表达水平均显著高于对照组(P0.05),另外,CD147、Cyp A在根尖囊肿中的表达明显高于在根尖肉芽肿中的表达(P=0.004、P=0.000)。CD147和Cyp A的蛋白表达水平在根尖肉芽肿(r=0.787,P0.05)和根尖囊肿(r=0.755,P0.05)中呈正相关;CD147和Cyp A的表达水平均与慢性根尖周炎的病变大小呈正相关(R2线性=0.746,R2线性=0.934,P0.05)。结论1.本实验通过免疫组织化学的方法,发现CD147在根尖肉芽肿和根尖囊肿中的蛋白表达水平均显著高于对照组,证实CD147存在于人慢性根尖周炎病损组织中。2.CD147、Cyp A在根尖囊肿中的表达明显高于在根尖肉芽肿中的表达,且CD147和Cyp A的蛋白表达水平在根尖囊肿和根尖肉芽肿中呈正相关,提示CD147和Cyp A可能参与根尖周病损的发生发展。3.CD147和Cyp A的表达水平均与慢性根尖周炎的病变大小呈正相关,说明CD147-Cyp A相互作用可能参与炎性反应和骨质吸收,且在人慢性根尖周病的发生发展过程中可能发挥了某种协同作用。
[Abstract]:Objective to investigate the expression of extracellular matrix metalloproteinase inducer CD147 EMMPRIN and cyclophilin A in human apical granuloma and apical cyst, and to explore the role of CD147 and Cyp A in the development of chronic apical periapical periodontitis. Methods from December 2014 to May 2015, dental and maxillofacial surgery was performed at the Oral and Maxillofacial Hospital of Anhui Medical University. The tissues of 35 cases of apical granuloma (35 cases) and 30 cases of apical cyst (30 cases) were identified by pathology as experimental group, and 8 cases of healthy alveolar bone were collected as normal control group. The mean periapical diameter was recorded by CBCT images as the periapical lesion size. The expression of CD147 and Cyp A in all samples was detected by immunohistochemical method. The expression levels of CD147 and Cyp A were analyzed according to the type of lesion (apical granuloma, cysts) and the size of periapical lesions. Results Immunohistochemical serial sections showed that cell CD147 and Cyp A were mainly expressed in inflammatory and epithelial regions, mainly lymphocytes, epithelial cells, macrophages, fibroblasts, and so on. CD147 and Cyp A were found in all periapical lesions. The expression of CD147 Cyp A was rare in normal control group. The positive expression of CD147 Cyp A in apical granuloma and apical cyst was significantly higher than that in control group (P0.05). In addition, the expression of CD147 Cyp A in apical cyst was significantly higher than that in apical granuloma. The expression levels of CD147 and Cyp A were positively correlated with the size of chronic periapical periodontitis (R2 linear 0.746R 2 linear 0.934 P 0.05) in apical granuloma (rn 0.787 P 0.05) and apical cysts (rn 0.755 P 0.05), and the expression levels of CD147 and Cyp A were positively correlated with the size of chronic periapical periodontitis (R 2 0. 746%, R 2 0. 746 0. 05 P 0.05), and the expression levels of CD147 and Cyp A were positively correlated with the size of chronic periapical periodontitis. Conclusion 1. The expression of CD147 protein in apical granuloma and apical cyst was significantly higher than that in control group by immunohistochemical method. The expression of CD147 in the lesions of chronic apical periodontitis was significantly higher than that in the apical granuloma, and the expression of CD147 and Cyp A was positively correlated with the apical cyst and apical granuloma. The results suggest that CD147 and Cyp A may be involved in the occurrence and development of periapical lesions. 3. The expression levels of CD147 and Cyp A are positively correlated with the size of chronic periapical disease, indicating that CD147-Cyp A interaction may be involved in inflammatory reaction and bone resorption. It may play a synergistic role in the occurrence and development of chronic periapical disease.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R781.341

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