人牙周膜干细胞条件培养液对舌癌Tca-8113细胞增殖及迁移能力的影响

发布时间：2018-06-29 22:13

本文选题：舌肿瘤 + 牙周膜　；参考：《肿瘤》2014年09期

【摘要】：目的 :探讨人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)条件培养液(conditioned medium,CM)对舌癌Tca-8113细胞增殖及迁移能力的影响。方法 :从健康成人牙周膜组织中分离并培养hPDLSCs。第3代hPDLSCs融合达80%时更换为无血清培养液,继续培养24 h后收集上清液,即获得hPDLSCs-CM。舌癌Tca-8113细胞分别在常规培养液(对照组)和含50%hPDLSCs-CM的培养液(CM处理组)中培养,然后采用MTT法检测细胞增殖活性,FCM法分析细胞周期变化,Transwell迁移实验检测细胞迁移能力的变化。结果 :分离培养的hPDLSCs表达干细胞标志蛋白Stro-1和CD146,且具有明显的克隆形成及多向分化能力。将hPDLSCs-CM与舌癌Tca-8113细胞共培养至第3、5和7天时,CM处理组的细胞增殖活性均高于未处理对照组(P0.01)。培养至第4天时,CM处理组的细胞增殖指数(proliferative index,PI)较对照组明显升高(P0.01)。培养24 h后,CM处理组穿膜细胞数明显高于对照组(P0.01)。结论 :hPDLSCs-CM可以促进舌癌Tca-8113细胞增殖,并增强该细胞的迁移能力。
[Abstract]:Objective: to investigate the effects of conditioned medium (conditioned medium CM) of human periodontal ligament stem cells (human periodontal ligament stem cells) on the proliferation and migration of tongue cancer Tca-8113 cells. Methods: hPDLSCswere isolated and cultured from periodontal ligament tissue of healthy adults. The third generation of hPDLSCs was replaced by serum-free medium when the fusion reached 80. The supernatant of hPDLSCs was collected after 24 hours of culture, that is, hPDLSCs-CM. Tca-8113 cells were cultured in conventional culture medium (control group) and culture medium containing 50 h PDLSCs-CM (CM treatment group), then cell cycle changes were detected by MTT assay and the cell migration ability was detected by Transwell migration assay. Results: hPDLSCs expressed Stro-1 and CD146, and had the ability of clone formation and multidirectional differentiation. The proliferative activity of hPDLSCs-CM and Tca-8113 cells was higher than that of untreated group (P0.01). At the 4th day of culture, the cell proliferation index (proliferative index Pi) in CM treated group was significantly higher than that in control group (P0.01). After 24 h culture, the number of perforating cells in CM treated group was significantly higher than that in control group (P0.01). Conclusion:% h PDLSCs-CM can promote the proliferation of Tca-8113 cells and enhance the migration ability of Tca-8113 cells.
【作者单位】：兰州大学口腔医学院修复科;
【基金】：国家自然科学基金青年科学基金项目(编号:81102712) 中央高校基本科研业务费专项资金项目(编号:861500)
【分类号】：R739.86

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