JNK抑制剂SP600125对人舌鳞癌Tca8113细胞增殖、迁移的影响

发布时间：2018-07-03 02:14

本文选题：SP600125 + Tca8113细胞　；参考：《延边大学》2017年硕士论文

【摘要】：目的:探讨JNK特定抑制剂SP600125对人舌鳞癌Tca8113细胞的增殖、迁移的影响及其机制。方法:用10、20、40μmol/L的JNK抑制剂SP600125处理人舌鳞癌Tca8113细胞24、48、72h,选用MTT法,检测SP600125对细胞增殖的抑制情况;选用细胞划痕实验检测SP600125对细胞迁移能力的影响。用10、20、40μmol/L的JNK抑制剂SP600125处理人舌鳞癌Tca8113细胞48h,选用免疫荧光实验,检测JNK和p-JNK蛋白的定位情况;选用Western blot,检测经SP600125处理后JNK和p-JNK蛋白的表达状况。结果:1.MTT结果显示:Tca8113细胞经10、20、40μmol/LSP600125分别处理24、48、72h后,与对照组相比,用药组的Tca8113细胞明显抑制增殖(P0.01);呈现出浓度和时间依赖性。2.划痕实验结果显示:Tca8113细胞经10、20、40μmol/L SP600125分别处理24、48、72h后,与对照组相比,用药组的细胞移动距离短于对照组(P0.01)。相同药物浓度时,用药时间越长,细胞移动距离越小;药物作用时间相同时,药物浓度越高,细胞移动距离越小。表明SP600125抑制Tca8113细胞的移动,呈现出浓度依赖性和时间依赖性。3.细胞免疫荧光染色结果显示:Tca8113细胞经10、20、40μmol/LSP600125处理48h,JNK蛋白主要定位在细胞质,用药组与对照组荧光密度未见明显变化;p-JNK蛋白主要定位在细胞质和细胞核内,用药组与对照组相比,随着用药浓度增加,其荧光密度减低。4.Western Blot结果显示:Tca8113细胞经10、20、40μmol/L P600125处理48h后提取蛋白,与对照组相比,经SP600125处理的Tca8113细胞中,p-JNK表达减少,且随着药物浓度的增加p-JNK表达越低(P0.01)。表明SP600125抑制p-JNK的表达,呈浓度依赖性。结论:SP600125明显抑制Tca8113细胞的增殖、迁移,SP600125的抗肿瘤机制可能与JNK有关。
[Abstract]:Objective: To investigate the effect of JNK specific inhibitor SP600125 on the proliferation and migration of Tca8113 cells in human tongue squamous cell carcinoma and its mechanism. Methods: the 24,48,72h of Tca8113 cells in human tongue squamous cell carcinoma was treated with JNK inhibitor SP600125 of 10,20,40 mol/L, and MTT method was used to detect the inhibition of cell proliferation, and the cell scratch test was used to detect the cells of the cells. The effect of migration ability. The Tca8113 cell 48h of human tongue squamous cell carcinoma was treated with the JNK inhibitor SP600125 of 10,20,40 micron mol/L. The localization of JNK and p-JNK protein was detected by immunofluorescence test. Western blot was used to detect the expression of JNK and protein after SP600125 treatment. 00125 after the treatment of 24,48,72h, compared with the control group, the Tca8113 cells in the control group significantly inhibited the proliferation (P0.01), and the results showed that the concentration and time dependent.2. scratch test showed that Tca8113 cells treated 24,48,72h after 10,20,40 mu mol/L SP600125 respectively, compared with the control group, the cell migration distance of the drug group was shorter than that of the control group (P0.01). At the same drug concentration, the longer the drug use time, the smaller the cell moving distance, the higher the drug concentration, the smaller the cell moving distance when the drug action time is the same. It shows that SP600125 inhibits the movement of Tca8113 cells, showing the concentration dependent and time dependent.3. cell immunofluorescence staining results show that Tca8113 cells are 10,20,40 mu mol/LSP600 125 treatment of 48h, JNK protein mainly located in the cytoplasm, the fluorescence density of the drug group and the control group did not change obviously, p-JNK protein was mainly located in the cytoplasm and nucleus. Compared with the control group, the drug group decreased with the increase of drug concentration, the fluorescence density of.4.Western Blot fruit showed that Tca8113 cells were treated by 10,20,40 mu mol/L P600125 48. After H extraction, the expression of p-JNK decreased in the Tca8113 cells treated with SP600125, and the expression of p-JNK decreased with the increase of drug concentration (P0.01). It indicated that SP600125 inhibited p-JNK expression in a concentration dependent manner. Conclusion: SP600125 obviously inhibits the proliferation of Tca8113 cells, migration, SP600125 anti-tumor mechanism may be associated with JNK. Close.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.86

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