单侧Ⅱ类牵引对成年大鼠颞下颌关节的滑膜的影响

发布时间：2018-07-08 21:38

  本文选题：牵引 + 颞下颌关节　； 参考：《郑州大学》2017年硕士论文

【摘要】：滑膜是颞下颌关节囊内软组织的重要组成成分,在维持关节的各种功能方面有重要作用。当有外界应力作用时,应力使口颌系统肌链的稳定状态发生改变,引起颞下颌关节周围肌肉的不平衡,使滑膜细胞在接受外界刺激信号后,直接或间接产生一些细胞因子,这些因子会引起自身和关节其他组织的适应、改建和破坏。IL-1β和VEGF是滑膜组织中常见的细胞因子,其超生理量的表达会介导一系列的化学反应,引起相应靶细胞的免疫反应。IL-1β是重要的促炎细胞因子之一,可直接和细胞膜上的受体结合,刺激各种胶原酶的生成,产生局部破坏作用,加重局部炎症反应;也可以通过诱导其他细胞因子的生成间接地参与关节的破坏机制。VEGF属于血小板生长因子家族,能特异性作用于血管内皮细胞,促使内皮细胞游走、增殖,在炎症部位形成新的血管,维持关节的炎症状态,使炎症部位发生增生、充血;也可以诱导和活化各种酶,改变血管通透性,促进多种炎症因子的释放等。目的:单侧Ⅱ类牵引改变了口颌系统肌链的稳定状态,引起颞下颌关节及其周围组织发生改变,通过观察和分析滑膜的组织学改变及IL-1β、VEGF表达的改变,来探讨单侧Ⅱ类牵引对成年大鼠牵引侧颞下颌关节滑膜的影响,进而为临床应用提供部分参考。方法:180只8周龄的SD(Sprague-Dawley)雄性大鼠,随机分为三组:实验组(A组),假手术组(B组),空白组(C组),每组60只。A组,在大鼠的右下第一磨牙和右上切牙之间固定Ni-Ti拉簧,加力20ɡ,建立单侧Ⅱ类牵引模型;B组,同A组固定拉簧,功能位加力0ɡ;C组,不固定拉簧。每组再按时间点(1d、3d、7d、14d、21d、28d)随机分为6个亚组,每亚组10只。按各亚组的时间点处死各组大鼠,取其右侧颞下颌关节双板区的滑膜组织进行HE、免疫组织化学染色,观察滑膜的组织学变化,检测IL-1β、VEGF的表达。结果:A组的病理分值和B组、C组比较有统计学意义(P0.001);A组的IL-1β、VEGF平均光密度值和B组、C组比较有统计学意义(P0.001)。病理分值与IL-1β、VEGF的MOD表达之间有显著正相关性(r1=0.912,r2=0.930;P0.001)。结论:单侧Ⅱ类牵引可引起牵引侧颞下颌关节滑膜组织发生组织学改变,这种改变与滑膜组织IL-1β、VEGF的表达有正相关性。
[Abstract]:Synovium is an important component of soft tissue in temporomandibular joint capsule and plays an important role in maintaining joint function. When there is external stress, the stress changes the stable state of the muscle chain of the oral and mandibular system, causes the imbalance of the muscles around the temporomandibular joint, and makes the synovial cells produce some cytokines directly or indirectly after receiving the external stimulation signal. These factors cause adaptation to themselves and other articular tissues. Remodeling and destroying. IL-1 尾 and VEGF are common cytokines in synovial tissue, and their superphysiological expression mediates a series of chemical reactions. IL-1 尾 is one of the important pro-inflammatory cytokines, which can directly bind to the receptors on the cell membrane, stimulate the production of various collagenase, produce local destruction and aggravate the local inflammatory reaction. VEGF belongs to the platelet growth factor family, which can specifically act on vascular endothelial cells, promote endothelial cell migration and proliferation, also can indirectly participate in the destruction mechanism of joint by inducing the formation of other cytokines. New blood vessels are formed in the inflammatory site to maintain the inflammatory state of the joint and make the inflammatory site proliferate and hyperemia. It can also induce and activate various enzymes change vascular permeability and promote the release of a variety of inflammatory factors. Objective: unilateral class 鈪，

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